Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 37708..38618 Replicon plasmid pKP2509-MCR
Accession NZ_CP065953
Organism Klebsiella pneumoniae strain KP2509

Toxin (Protein)


Gene name - Uniprot ID A0A1D8K2L2
Locus tag JDE60_RS28385 Protein ID WP_070083042.1
Coordinates 38148..38618 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag JDE60_RS28380 Protein ID WP_004181895.1
Coordinates 37708..38151 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDE60_RS28355 (JDE60_28335) 32864..33079 - 216 Protein_43 IS200/IS605 family transposase -
JDE60_RS28360 (JDE60_28340) 33081..33650 + 570 Protein_44 transposase -
JDE60_RS28365 (JDE60_28345) 34018..35505 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
JDE60_RS28370 (JDE60_28350) 35914..36336 + 423 WP_135717959.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
JDE60_RS28375 (JDE60_28355) 36336..37607 + 1272 WP_004197886.1 translesion error-prone DNA polymerase V subunit UmuC -
JDE60_RS28380 (JDE60_28360) 37708..38151 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
JDE60_RS28385 (JDE60_28365) 38148..38618 + 471 WP_070083042.1 RES family NAD+ phosphorylase Toxin
JDE60_RS28390 (JDE60_28370) 38729..38989 - 261 WP_070083041.1 hypothetical protein -
JDE60_RS28395 (JDE60_28375) 39779..41119 + 1341 WP_000589001.1 ISNCY family transposase -
JDE60_RS28400 (JDE60_28380) 41762..42334 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
JDE60_RS28405 (JDE60_28385) 42534..43457 + 924 WP_000167917.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IIa / blaTEM-1B / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / mcr-1.1 - 1..317010 317010
- flank IS/Tn - - 33297..33650 353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17587.85 Da        Isoelectric Point: 4.5244

>T183685 WP_070083042.1 NZ_CP065953:38148-38618 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMESFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T183685 NZ_CP087152:c41044-40733 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT183685 WP_004181895.1 NZ_CP065953:37708-38151 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT183685 NZ_CP087152:c40736-40317 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K2L2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References