Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2772705..2774293 Replicon chromosome
Accession NZ_CP065949
Organism Klebsiella pneumoniae strain KP2509

Toxin (Protein)


Gene name hipA Uniprot ID A0A0H3GN97
Locus tag JDE60_RS13875 Protein ID WP_004199851.1
Coordinates 2772705..2774027 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag JDE60_RS13880 Protein ID WP_002904790.1
Coordinates 2774027..2774293 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDE60_RS13860 (JDE60_13860) 2767770..2770271 + 2502 WP_004199853.1 fimbria/pilus outer membrane usher protein -
JDE60_RS13865 (JDE60_13865) 2770259..2771263 + 1005 WP_004199852.1 fimbrial protein -
JDE60_RS13870 (JDE60_13870) 2771396..2772688 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
JDE60_RS13875 (JDE60_13875) 2772705..2774027 - 1323 WP_004199851.1 type II toxin-antitoxin system HipA family toxin Toxin
JDE60_RS13880 (JDE60_13880) 2774027..2774293 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
JDE60_RS13885 (JDE60_13885) 2774559..2774885 - 327 WP_002904788.1 hydroxyisourate hydrolase -
JDE60_RS13890 (JDE60_13890) 2774882..2775382 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
JDE60_RS13895 (JDE60_13895) 2775360..2776739 - 1380 WP_004199850.1 nucleobase:cation symporter-2 family protein -
JDE60_RS13900 (JDE60_13900) 2777085..2778239 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
JDE60_RS13905 (JDE60_13905) 2778220..2779143 - 924 WP_004199849.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49128.44 Da        Isoelectric Point: 7.2487

>T183677 WP_004199851.1 NZ_CP065949:c2774027-2772705 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T183677 NZ_CP087151:2008295-2008397 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT183677 WP_002904790.1 NZ_CP065949:c2774293-2774027 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT183677 NZ_CP087151:c2008404-2008259 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GN97

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References