Detailed information of TA system TA183668

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 889842..890496
• Replicon: chromosome
• Accession: NZ_CP065949
• Organism: Klebsiella pneumoniae strain KP2509

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R4YHX2
• Locus tag: JDE60_RS04520
• Protein ID: WP_004144731.1
• Coordinates: 890089..890496 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: JDE60_RS04515
• Protein ID: WP_002916312.1
• Coordinates: 889842..890108 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDE60_RS04490 (JDE60_04490)	884998..886431	-	1434	WP_002916322.1	6-phospho-beta-glucosidase BglA	-
JDE60_RS04495 (JDE60_04495)	886550..887278	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
JDE60_RS04500 (JDE60_04500)	887328..887639	+	312	WP_002916319.1	N(4)-acetylcytidine aminohydrolase	-
JDE60_RS04505 (JDE60_04505)	887803..888462	+	660	WP_002916317.1	hemolysin III family protein	-
JDE60_RS04510 (JDE60_04510)	888613..889596	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
JDE60_RS04515 (JDE60_04515)	889842..890108	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
JDE60_RS04520 (JDE60_04520)	890089..890496	+	408	WP_004144731.1	protein YgfX	Toxin
JDE60_RS04525 (JDE60_04525)	890503..891024	-	522	WP_002916308.1	flavodoxin FldB	-
JDE60_RS04530 (JDE60_04530)	891125..892021	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
JDE60_RS04535 (JDE60_04535)	892044..892757	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
JDE60_RS04540 (JDE60_04540)	892763..894496	+	1734	WP_004151783.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15936.69 Da
• Isoelectric Point: 11.4778

>T183668 WP_004144731.1 NZ_CP065949:890089-890496 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 408 bp

>T183668 NZ_CP087147:c41044-40733 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT183668 WP_002916312.1 NZ_CP065949:889842-890108 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT183668 NZ_CP087147:c40736-40317 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A378E4P6

Antitoxin

• AlphaFold DB: A0A0H3GY41