Detailed information of TA system TA183662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 3220161..3221419
• Replicon: chromosome
• Accession: NZ_CP065948
• Organism: Pseudomonas aeruginosa strain PAM68

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JBL37_RS15125
• Protein ID: WP_023084953.1
• Coordinates: 3220161..3220916 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: JBL37_RS15130
• Protein ID: WP_003086329.1
• Coordinates: 3221051..3221419 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JBL37_RS15115 (JBL37_15120)	3215334..3217877	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
JBL37_RS15120 (JBL37_15125)	3218032..3219393	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
JBL37_RS15125 (JBL37_15130)	3220161..3220916	-	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
JBL37_RS15130 (JBL37_15135)	3221051..3221419	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
JBL37_RS15135 (JBL37_15140)	3221492..3222778	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
JBL37_RS15140 (JBL37_15145)	3222834..3224327	-	1494	WP_282309516.1	aldehyde dehydrogenase family protein	-
JBL37_RS15145 (JBL37_15150)	3224501..3224977	+	477	WP_009877993.1	elongation factor GreAB	-
JBL37_RS15150 (JBL37_15155)	3224980..3225255	-	276	WP_023088486.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T183662 WP_023084953.1 NZ_CP065948:c3220916-3220161 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T183662 NZ_CP087146:4195829-4196047 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT183662 WP_003086329.1 NZ_CP065948:c3221419-3221051 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT183662 NZ_CP087146:4195428-4195802 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: V6AJU7