Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 624066..625574 Replicon chromosome
Accession NZ_CP065948
Organism Pseudomonas aeruginosa strain PAM68

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag JBL37_RS02855 Protein ID WP_003119682.1
Coordinates 624066..624653 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1C7C4U7
Locus tag JBL37_RS02860 Protein ID WP_003091607.1
Coordinates 624720..625574 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JBL37_RS02850 (JBL37_02855) 620321..623800 - 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
JBL37_RS02855 (JBL37_02860) 624066..624653 + 588 WP_003119682.1 GNAT family protein Toxin
JBL37_RS02860 (JBL37_02865) 624720..625574 + 855 WP_003091607.1 AraC family transcriptional regulator Antitoxin
JBL37_RS02865 (JBL37_02870) 625671..627836 + 2166 WP_003115567.1 TonB-dependent receptor family protein -
JBL37_RS02870 (JBL37_02875) 628011..629885 + 1875 WP_003117607.1 MFS transporter -
JBL37_RS02875 (JBL37_02880) 629946..630155 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T183660 WP_003119682.1 NZ_CP065948:624066-624653 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T183660 NZ_CP087146:2008295-2008397 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31895.20 Da        Isoelectric Point: 7.5295

>AT183660 WP_003091607.1 NZ_CP065948:624720-625574 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Download         Length: 855 bp

>AT183660 NZ_CP087146:c2008404-2008259 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C4U7

References