Detailed information of TA system TA183659

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 5174164..5175385
• Replicon: chromosome
• Accession: NZ_CP065947
• Organism: Pseudomonas aeruginosa strain PAS6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: JBL36_RS24520
• Protein ID: WP_003102740.1
• Coordinates: 5174164..5174742 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JBL36_RS24525
• Protein ID: WP_003088112.1
• Coordinates: 5174831..5175385 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JBL36_RS24505 (JBL36_24500)	5169440..5170717	+	1278	WP_003122808.1	TolC family protein	-
JBL36_RS24510 (JBL36_24505)	5170707..5172878	+	2172	WP_003114950.1	type I secretion system permease/ATPase	-
JBL36_RS24515 (JBL36_24510)	5172868..5174055	+	1188	WP_105248280.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JBL36_RS24520 (JBL36_24515)	5174164..5174742	-	579	WP_003102740.1	HD domain-containing protein	Toxin
JBL36_RS24525 (JBL36_24520)	5174831..5175385	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
JBL36_RS24530 (JBL36_24525)	5175543..5177738	-	2196	WP_003114948.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
JBL36_RS24535 (JBL36_24530)	5177743..5178204	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
JBL36_RS24540 (JBL36_24535)	5178535..5178858	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
JBL36_RS24545 (JBL36_24540)	5178871..5179269	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
JBL36_RS24550 (JBL36_24545)	5179266..5179817	-	552	WP_003132721.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T183659 WP_003102740.1 NZ_CP065947:c5174742-5174164 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T183659 NZ_CP087146:876028-876435 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT183659 WP_003088112.1 NZ_CP065947:c5175385-5174831 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT183659 NZ_CP087146:875781-876047 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07