Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 94282..95905 Replicon plasmid unnamed1
Accession NZ_CP065940
Organism Enterobacter hormaechei strain MSB1_8J

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag JDC97_RS24090 Protein ID WP_000088044.1
Coordinates 94580..95905 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag JDC97_RS24085 Protein ID WP_012477377.1
Coordinates 94282..94575 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDC97_RS24065 90081..90473 + 393 WP_000341066.1 hypothetical protein -
JDC97_RS24070 91132..91788 + 657 WP_000462754.1 trypsin-like serine protease -
JDC97_RS24075 91991..92488 + 498 WP_000340139.1 membrane protein -
JDC97_RS24080 92493..93881 + 1389 WP_012695445.1 hypothetical protein -
JDC97_RS24085 94282..94575 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
JDC97_RS24090 94580..95905 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
JDC97_RS24095 95966..96172 + 207 WP_000134171.1 hypothetical protein -
JDC97_RS24100 96273..96683 + 411 WP_000985909.1 hypothetical protein -
JDC97_RS24105 96696..97124 + 429 Protein_92 hypothetical protein -
JDC97_RS24110 97402..98406 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
JDC97_RS24115 98497..98931 - 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / formA / mcr-9 / tet(D) / blaSHV-12 / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) - 1..307080 307080
- flank IS/Tn - - 97402..98406 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T183643 WP_000088044.1 NZ_CP065940:94580-95905 [Enterobacter hormaechei]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T183643 NZ_CP087142:2010305-2010407 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT183643 WP_012477377.1 NZ_CP065940:94282-94575 [Enterobacter hormaechei]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT183643 NZ_CP087142:c2010414-2010269 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References