Detailed information of TA system TA183628

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 665952..666655
• Replicon: chromosome
• Accession: NZ_CP065939
• Organism: Enterobacter hormaechei strain MSB1_8J

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8B5ZZ18
• Locus tag: JDC97_RS03230
• Protein ID: WP_039268718.1
• Coordinates: 666314..666655 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8B5ZWQ9
• Locus tag: JDC97_RS03225
• Protein ID: WP_039268717.1
• Coordinates: 665952..666293 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDC97_RS03190	661105..661980	+	876	WP_039268712.1	GTPase family protein	-
JDC97_RS03195	662224..662940	+	717	WP_039268713.1	WYL domain-containing protein	-
JDC97_RS03200	662976..663428	+	453	WP_039269148.1	hypothetical protein	-
JDC97_RS03205	663500..663973	+	474	WP_039268714.1	hypothetical protein	-
JDC97_RS03210	664094..664915	+	822	WP_039268715.1	DUF945 domain-containing protein	-
JDC97_RS03215	664946..665386	+	441	WP_039268716.1	antirestriction protein	-
JDC97_RS03220	665399..665941	+	543	WP_045259572.1	DNA repair protein RadC	-
JDC97_RS03225	665952..666293	+	342	WP_039268717.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JDC97_RS03230	666314..666655	+	342	WP_039268718.1	TA system toxin CbtA family protein	Toxin
JDC97_RS03235	666910..667917	+	1008	WP_039268719.1	restriction endonuclease	-
JDC97_RS03240	668098..668934	-	837	Protein_625	HNH endonuclease	-
JDC97_RS03245	669234..669701	+	468	WP_022650322.1	SymE family type I addiction module toxin	-
JDC97_RS03250	669759..670130	-	372	WP_022650323.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	619396..674601	55205

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-112)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12772.83 Da
• Isoelectric Point: 9.6548

>T183628 WP_039268718.1 NZ_CP065939:666314-666655 [Enterobacter hormaechei]
MKTLPATTPQAAKLCLLPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T183628 NZ_CP087136:c3226984-3226670 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12729.40 Da
• Isoelectric Point: 6.9460

>AT183628 WP_039268717.1 NZ_CP065939:665952-666293 [Enterobacter hormaechei]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRVSITGKFSEAECLKLDVAFPHFISQMESMLTTGGMNPRH
AHCVTLYHNGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 342 bp

>AT183628 NZ_CP087136:c3226673-3226257 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A8B5ZZ18

Antitoxin

• AlphaFold DB: A0A8B5ZWQ9