Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 15659..16266 Replicon plasmid p5_56kb
Accession NZ_CP065931
Organism Enterococcus faecium strain Dallas 5

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag JCP67_RS15010 Protein ID WP_002321032.1
Coordinates 15916..16266 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag JCP67_RS15005 Protein ID WP_002287514.1
Coordinates 15659..15922 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCP67_RS14965 11009..11482 + 474 WP_002321302.1 single-stranded DNA-binding protein -
JCP67_RS14970 11848..12108 + 261 WP_002321303.1 hypothetical protein -
JCP67_RS14975 12553..12759 + 207 WP_002296239.1 hypothetical protein -
JCP67_RS14980 12759..13010 + 252 WP_002305766.1 hypothetical protein -
JCP67_RS14985 13026..13442 + 417 WP_002305764.1 hypothetical protein -
JCP67_RS14990 13424..13696 + 273 WP_002348912.1 hypothetical protein -
JCP67_RS14995 13863..14264 + 402 WP_002305759.1 IS200/IS605 family transposase -
JCP67_RS15000 14276..15421 + 1146 WP_002305757.1 IS200/IS605 family element transposase accessory protein TnpB -
JCP67_RS15005 15659..15922 + 264 WP_002287514.1 hypothetical protein Antitoxin
JCP67_RS15010 15916..16266 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JCP67_RS15015 16290..16904 - 615 WP_002318230.1 recombinase family protein -
JCP67_RS15020 17218..17640 + 423 WP_002288787.1 hypothetical protein -
JCP67_RS15025 17650..17853 + 204 WP_002299573.1 hypothetical protein -
JCP67_RS15030 18064..18648 + 585 WP_002299575.1 recombinase family protein -
JCP67_RS15035 18817..20142 + 1326 WP_016171137.1 Y-family DNA polymerase -
JCP67_RS15040 20135..20485 + 351 WP_002303114.1 hypothetical protein -
JCP67_RS15045 20482..20661 + 180 WP_002322386.1 hypothetical protein -
JCP67_RS15050 20673..20966 + 294 WP_002322251.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..56294 56294


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T183620 WP_002321032.1 NZ_CP065931:15916-16266 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T183620 NZ_CP087136:2071597-2071971 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT183620 WP_002287514.1 NZ_CP065931:15659-15922 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT183620 NZ_CP087136:2071140-2071508 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References