Detailed information of TA system TA183611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2244749..2245284
• Replicon: chromosome
• Accession: NZ_CP065925
• Organism: Staphylococcus pseudintermedius strain SP_11304-1A

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A2A4EJD1
• Locus tag: JC282_RS10725
• Protein ID: WP_015728663.1
• Coordinates: 2244946..2245284 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: JC282_RS10720
• Protein ID: WP_198455020.1
• Coordinates: 2244749..2244949 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC282_RS10695	2240016..2240621	-	606	WP_014614680.1	DUF2812 domain-containing protein	-
JC282_RS10700	2240605..2240937	-	333	WP_096548189.1	PadR family transcriptional regulator	-
JC282_RS10705	2241090..2242631	-	1542	WP_014614682.1	SLC13 family permease	-
JC282_RS10710	2243211..2243672	-	462	WP_014614683.1	threonine/serine exporter family protein	-
JC282_RS10715	2243685..2244440	-	756	WP_014614684.1	threonine/serine exporter family protein	-
JC282_RS10720	2244749..2244949	+	201	WP_198455020.1	AbrB family transcriptional regulator	Antitoxin
JC282_RS10725	2244946..2245284	+	339	WP_015728663.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JC282_RS10730	2245492..2246247	-	756	WP_014614687.1	heme-dependent peroxidase	-
JC282_RS10735	2246415..2247404	+	990	WP_198455021.1	phosphate acetyltransferase	-
JC282_RS10740	2247404..2248240	+	837	WP_015728660.1	lipoate--protein ligase family protein	-
JC282_RS10745	2248394..2248591	-	198	Protein_2081	IS200/IS605 family transposase	-
JC282_RS10750	2248835..2249185	+	351	WP_049770159.1	S8 family serine peptidase	-
JC282_RS10755	2249188..2249937	+	750	WP_198455022.1	SAR2788 family putative toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13034.20 Da
• Isoelectric Point: 9.6922

>T183611 WP_015728663.1 NZ_CP065925:2244946-2245284 [Staphylococcus pseudintermedius]
MSIKQFDIIYIDLNPTRGREKQNIRPCLVINNQMMIDGTELVWVVPIINRELRYPTDIELKTKKGLVSGVIDSIQIRALD
LNARQHHYKDALQDNLKSKVLKAIQTYLNPNY

Nucleotide

• Length: 339 bp

>T183611 NZ_CP087136:c476130-475912 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7389.27 Da
• Isoelectric Point: 4.3776

>AT183611 WP_198455020.1 NZ_CP065925:2244749-2244949 [Staphylococcus pseudintermedius]
MSLSKKTIQSVDFEIGDTVEVQKINGGLFITKKEVSIEDSIKNFFQNGGKYQESEVEFGESVGREI

Nucleotide

• Length: 201 bp

>AT183611 NZ_CP087136:c476530-476156 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2A4EJD1