Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 757639..758163 Replicon chromosome
Accession NZ_CP065925
Organism Staphylococcus pseudintermedius strain SP_11304-1A

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag JC282_RS03390 Protein ID WP_014613379.1
Coordinates 757810..758163 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag JC282_RS03385 Protein ID WP_014613378.1
Coordinates 757639..757809 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC282_RS03360 753528..754001 + 474 WP_014613373.1 PH domain-containing protein -
JC282_RS03365 753994..755523 + 1530 WP_130921433.1 PH domain-containing protein -
JC282_RS03370 755507..756025 + 519 WP_014613375.1 PH domain-containing protein -
JC282_RS03375 756022..756372 + 351 WP_014613376.1 holo-ACP synthase -
JC282_RS03380 756406..757554 + 1149 WP_014613377.1 alanine racemase -
JC282_RS03385 757639..757809 + 171 WP_014613378.1 hypothetical protein Antitoxin
JC282_RS03390 757810..758163 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JC282_RS03395 758221..759225 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
JC282_RS03400 759304..759630 + 327 WP_014613381.1 anti-sigma factor antagonist -
JC282_RS03405 759630..760109 + 480 WP_180932480.1 anti-sigma B factor RsbW -
JC282_RS03410 760084..760854 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T183610 WP_014613379.1 NZ_CP065925:757810-758163 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T183610 NZ_CP087136:c259380-259039 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT183610 WP_014613378.1 NZ_CP065925:757639-757809 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT183610 NZ_CP087136:c259718-259401 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References