Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 863869..864393 Replicon chromosome
Accession NZ_CP065924
Organism Staphylococcus pseudintermedius strain SP_11304-2A

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag JC285_RS03870 Protein ID WP_014613379.1
Coordinates 864040..864393 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag JC285_RS03865 Protein ID WP_014613378.1
Coordinates 863869..864039 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC285_RS03840 859758..860231 + 474 WP_014613373.1 PH domain-containing protein -
JC285_RS03845 860224..861753 + 1530 WP_198462656.1 PH domain-containing protein -
JC285_RS03850 861737..862255 + 519 WP_110179271.1 PH domain-containing protein -
JC285_RS03855 862252..862602 + 351 WP_198462657.1 holo-ACP synthase -
JC285_RS03860 862636..863784 + 1149 WP_143951660.1 alanine racemase -
JC285_RS03865 863869..864039 + 171 WP_014613378.1 hypothetical protein Antitoxin
JC285_RS03870 864040..864393 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JC285_RS03875 864451..865455 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
JC285_RS03880 865534..865860 + 327 WP_014613381.1 anti-sigma factor antagonist -
JC285_RS03885 865860..866339 + 480 WP_031253870.1 anti-sigma B factor RsbW -
JC285_RS03890 866314..867084 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T183607 WP_014613379.1 NZ_CP065924:864040-864393 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T183607 NZ_CP087132:2869010-2869294 [Flavobacterium sp. F-29]
ATGGAAACGTCAAAATTTGATATTGCAGATTACTTGGACAGCAACGAAATGATTGCTGAATATCTAAATACCGTTTTGGA
AGAAGGCAATGATGCTGAAATTGTTACTGCAATTGGACATATTGCAAAAGCAATTGGTATGACCAAAATTGCAGAAGAAA
CTAGAATGAGCAGACCAAGTTTGTACAAAGCATTGTCGGAAGGATCCAAACCACAATTTTCAACGATAATGAAAGTTTTG
AAAGCAATTGGAGGACAAATACATATAAATCCATTAACCTCATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT183607 WP_014613378.1 NZ_CP065924:863869-864039 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT183607 NZ_CP087132:2868707-2868886 [Flavobacterium sp. F-29]
ATGTACTTTATTGAAAAGACGACGGAATTTGATAAGTGGTTTAAGAAATTGAATGACTTTAGAGCAAAAGCAAAAATTTT
GTTTCGACTTCAAAAGTTAGAAAAGGACGAACACTTTGGAGATTGCGAACCTGTTGGAGATGGAATTAGAGAACTAAAAA
TCAACTACGCAAAAGGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References