Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 808962..809483 Replicon chromosome
Accession NZ_CP065921
Organism Staphylococcus pseudintermedius strain SP_11304-3A

Toxin (Protein)


Gene name TsdT Uniprot ID A0A3D8YLE8
Locus tag JC286_RS03565 Protein ID WP_014613364.1
Coordinates 809262..809483 (+) Length 74 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID A0A166R1D0
Locus tag JC286_RS03560 Protein ID WP_014613363.1
Coordinates 808962..809249 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC286_RS03535 804223..805026 + 804 WP_014613358.1 hydroxyethylthiazole kinase -
JC286_RS03540 805016..805654 + 639 WP_014613359.1 thiamine phosphate synthase -
JC286_RS03545 805743..806609 + 867 WP_014613360.1 membrane protein insertase YidC -
JC286_RS03550 806663..807319 - 657 WP_014613361.1 HD domain-containing protein -
JC286_RS03555 807340..808830 - 1491 WP_014613362.1 cardiolipin synthase -
JC286_RS03560 808962..809249 + 288 WP_014613363.1 copper-sensing transcriptional repressor CsoR Antitoxin
JC286_RS03565 809262..809483 + 222 WP_014613364.1 heavy-metal-associated domain-containing protein Toxin
JC286_RS03570 809487..809693 + 207 WP_014613365.1 cation transporter -
JC286_RS03575 809794..811974 + 2181 WP_014613366.1 heavy metal translocating P-type ATPase -
JC286_RS03580 812060..812206 + 147 WP_014613367.1 hypothetical protein -
JC286_RS03585 813173..813901 + 729 WP_014613368.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(21-64)

Antitoxin

Metal-sensitive transcriptional repressor

(3-93)


Sequences

Toxin        


Download         Length: 74 a.a.        Molecular weight: 8065.10 Da        Isoelectric Point: 4.3480

>T183603 WP_014613364.1 NZ_CP065921:809262-809483 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGFPVIQSHKGV

Download         Length: 222 bp

>T183603 NZ_CP087123:5570379-5570690 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10997.59 Da        Isoelectric Point: 7.0504

>AT183603 WP_014613363.1 NZ_CP065921:808962-809249 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Download         Length: 288 bp

>AT183603 NZ_CP087123:5570687-5571106 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3D8YLE8

Antitoxin

Source ID Structure
AlphaFold DB A0A166R1D0

References