Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 814079..814603 Replicon chromosome
Accession NZ_CP065919
Organism Staphylococcus pseudintermedius strain SP_11306-4A

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag JC284_RS03600 Protein ID WP_014613379.1
Coordinates 814250..814603 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag JC284_RS03595 Protein ID WP_014613378.1
Coordinates 814079..814249 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JC284_RS03570 809968..810441 + 474 WP_014613373.1 PH domain-containing protein -
JC284_RS03575 810434..811963 + 1530 WP_198458615.1 PH domain-containing protein -
JC284_RS03580 811947..812465 + 519 WP_014613375.1 PH domain-containing protein -
JC284_RS03585 812462..812812 + 351 WP_014613376.1 holo-ACP synthase -
JC284_RS03590 812846..813994 + 1149 WP_014613377.1 alanine racemase -
JC284_RS03595 814079..814249 + 171 WP_014613378.1 hypothetical protein Antitoxin
JC284_RS03600 814250..814603 + 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JC284_RS03605 814661..815665 + 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
JC284_RS03610 815744..816070 + 327 WP_014613381.1 anti-sigma factor antagonist -
JC284_RS03615 816070..816549 + 480 WP_031253870.1 anti-sigma B factor RsbW -
JC284_RS03620 816524..817294 + 771 WP_015729428.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T183598 WP_014613379.1 NZ_CP065919:814250-814603 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T183598 NZ_CP087123:c4748417-4747884 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT183598 WP_014613378.1 NZ_CP065919:814079-814249 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT183598 NZ_CP087123:c4748694-4748428 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References