Detailed information of TA system TA183597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 797672..798193
• Replicon: chromosome
• Accession: NZ_CP065919
• Organism: Staphylococcus pseudintermedius strain SP_11306-4A

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: JC284_RS03520
• Protein ID: WP_110178839.1
• Coordinates: 797972..798193 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A166R1D0
• Locus tag: JC284_RS03515
• Protein ID: WP_014613363.1
• Coordinates: 797672..797959 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JC284_RS03490	792933..793736	+	804	WP_014613358.1	hydroxyethylthiazole kinase	-
JC284_RS03495	793726..794364	+	639	WP_014613359.1	thiamine phosphate synthase	-
JC284_RS03500	794453..795319	+	867	WP_014613360.1	membrane protein insertase YidC	-
JC284_RS03505	795373..796029	-	657	WP_014613361.1	HD domain-containing protein	-
JC284_RS03510	796050..797540	-	1491	WP_014613362.1	cardiolipin synthase	-
JC284_RS03515	797672..797959	+	288	WP_014613363.1	copper-sensing transcriptional repressor CsoR	Antitoxin
JC284_RS03520	797972..798193	+	222	WP_110178839.1	heavy-metal-associated domain-containing protein	Toxin
JC284_RS03525	798197..798403	+	207	WP_014613365.1	cation transporter	-
JC284_RS03530	798504..800684	+	2181	WP_131091343.1	heavy metal translocating P-type ATPase	-
JC284_RS03535	800770..800916	+	147	WP_014613367.1	hypothetical protein	-
JC284_RS03540	801884..802612	+	729	WP_198458614.1	peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (21-64)

Antitoxin

• Metal-sensitive transcriptional repressor (3-93)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8081.10 Da
• Isoelectric Point: 4.3480

>T183597 WP_110178839.1 NZ_CP065919:797972-798193 [Staphylococcus pseudintermedius]
MENGYVKVTQLTTEAQQQQLSDRLLRLIGVESVAIDVATQSLTLTYDTPANLNTIEKEIYDAGYPVIQSHKGV

Nucleotide

• Length: 222 bp

>T183597 NZ_CP087123:4157019-4157237 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10997.59 Da
• Isoelectric Point: 7.0504

>AT183597 WP_014613363.1 NZ_CP065919:797672-797959 [Staphylococcus pseudintermedius]
MTEQAHHSEEIKRNLKSRLNRIEGQVKAINRMIDEDVYCDDVLTQIRATRSALNSVASRLLDHHMKGCIKHKIDAGHQDE
AMEELLVTFQKLMKD

Nucleotide

• Length: 288 bp

>AT183597 NZ_CP087123:4156618-4156992 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A166R1D0