Detailed information of TA system TA183573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1640480..1642175
• Replicon: chromosome
• Accession: NZ_CP065867
• Organism: Pseudomonas aeruginosa strain TJ2014-049

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: I9X29_RS07685
• Protein ID: WP_198341234.1
• Coordinates: 1640480..1641349 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A1C7C9F9
• Locus tag: I9X29_RS07690
• Protein ID: WP_017001846.1
• Coordinates: 1641342..1642175 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X29_RS07660	1636488..1637339	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
I9X29_RS07665	1637380..1638387	+	1008	WP_023106073.1	ABC transporter substrate-binding protein	-
I9X29_RS07670	1638384..1639160	+	777	WP_003112898.1	ABC transporter permease	-
I9X29_RS07675	1639166..1639927	+	762	WP_198341233.1	SDR family oxidoreductase	-
I9X29_RS07680	1639941..1640471	+	531	WP_003112900.1	cupin domain-containing protein	-
I9X29_RS07685	1640480..1641349	+	870	WP_198341234.1	alpha/beta hydrolase	Toxin
I9X29_RS07690	1641342..1642175	+	834	WP_017001846.1	IclR family transcriptional regulator	Antitoxin
I9X29_RS07695	1642162..1642959	+	798	WP_003112903.1	SDR family oxidoreductase	-
I9X29_RS07700	1642952..1644634	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
I9X29_RS07705	1644645..1645448	+	804	WP_003453564.1	aspartate dehydrogenase	-
I9X29_RS07710	1645460..1646944	+	1485	WP_198341235.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30336.60 Da
• Isoelectric Point: 6.5894

>T183573 WP_198341234.1 NZ_CP065867:1640480-1641349 [Pseudomonas aeruginosa]
MNMAQTHIPAPSLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQCLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAARLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERLGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSPIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T183573 NZ_CP087114:c1345158-1344958 [Bartonella machadoae]
ATGGAACAAGATAGCCGAAAAATCATTGCAAAATTAAAGCGTGATGGCTTTGAACTTGTGAAAGTGAGAGGTTCTCATCA
TAAATTAAAAAAAGATGATAAAGTTGTTATTGTCCCACATCCTAAAAAAAATCTTCCTATTGGTACAGCACGTTCTATTG
CGCAACAAGCAGGCTGGTTGAAAAAAGGAGAAGAAGAATGA

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30867.22 Da
• Isoelectric Point: 5.7725

>AT183573 WP_017001846.1 NZ_CP065867:1641342-1642175 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRSHETV

Nucleotide

• Length: 834 bp

>AT183573 NZ_CP087114:c1344961-1344566 [Bartonella machadoae]
ATGAAAAGATTTTTTGCTCTTGTTCATAAAGATGAAGATTCTGCTTTTGGTGTTCAGTTTCCTGACTTTGAAGGACTTTT
TTCTGCTGCGGATGAAGAAAAAAATCTCATCACAAATGCAACTGAAGCCCTTCAACTTTATTGTGAAGATATGGATACAG
TGCCTATGCCCTTAAAATTTGAAGAAGTCATACAACAGCAATCTGTCAAAACAGCTTTGGCAGAAGGAGCTTTTTTAATA
CAAGTCCCCTTTATTGAAAATGATGCTGAAGTGGTACGTATGAATATATCTATTGAACGAGGACTTTTACGTGCCATTGA
TAATTGTGCACAAGAAAGAGGCTTAACAAGATCTGCTTTTTTGGCAACAGCAGCGCGTCACGAGCTTAATATTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1C7C9F9