Detailed information of TA system TA183569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4482368..4483626
• Replicon: chromosome
• Accession: NZ_CP065866
• Organism: Pseudomonas aeruginosa strain TJ2019-017

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: I9X27_RS20640
• Protein ID: WP_023084953.1
• Coordinates: 4482368..4483123 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: I9X27_RS20645
• Protein ID: WP_003086329.1
• Coordinates: 4483258..4483626 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X27_RS20630	4477541..4480084	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
I9X27_RS20635	4480239..4481600	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
I9X27_RS20640	4482368..4483123	-	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
I9X27_RS20645	4483258..4483626	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
I9X27_RS20650	4483699..4484985	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
I9X27_RS20655	4485041..4486630	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
I9X27_RS20660	4486709..4487185	+	477	WP_023122241.1	hypothetical protein	-
I9X27_RS20665	4487188..4487463	-	276	WP_171945305.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T183569 WP_023084953.1 NZ_CP065866:c4483123-4482368 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T183569 NZ_CP087114:1159778-1160059 [Bartonella machadoae]
ATGCGGACGATTGAACGTACCACTATCTTTAAACGTGATTTTAAGCGTGAAATGAGAGGGCGATATCGGCATCTTTTAGA
GACTGACTTGCGCCAAATTATTGCAGCATTAGCAAATGATCAACCTTTAGAACCTCGACATCATGATCATGCATTAACTG
GAAATTGGAAAGATTATCGAGATTGCCATATTCGACCTGATTTGGTTTTGATTTATCGATTAATTGGTCAAGATAGACTT
ATTTTGGTGCGTCTTGGTTCTCATTCACAGCTTAATCTGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT183569 WP_003086329.1 NZ_CP065866:c4483626-4483258 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT183569 NZ_CP087114:1159528-1159791 [Bartonella machadoae]
ATGGCTACCAGTCGCATGGTGCAAGCGCGTGTACCAGAAGAGATCCAAAATGTCGCTAATCAAGTTATTCAAGCCTCTGG
TTTATCGGTGAGTGATGTTGTGAGAGTGTTGATGACGCGTATTGCGCAAGATAAAGCTATTCCGTCCTTTTTATTTCAAC
CCAATGCAGAGACCATAGCGGCTTTTACTGAATTAGACGAAGGCAACTTAAAAAAGTTTAATTCTGTAGACGAATTATTT
GATGATTTATATGCGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7