Detailed information of TA system TA183565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 141504..142009
• Replicon: chromosome
• Accession: NZ_CP065866
• Organism: Pseudomonas aeruginosa strain TJ2019-017

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: I9X27_RS00645
• Protein ID: WP_003083773.1
• Coordinates: 141504..141785 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: I9X27_RS00650
• Protein ID: WP_003083775.1
• Coordinates: 141782..142009 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X27_RS00620	136755..138104	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
I9X27_RS00625	138153..138839	+	687	WP_003083762.1	FadR family transcriptional regulator	-
I9X27_RS00630	138940..139674	+	735	WP_023119575.1	GntR family transcriptional regulator	-
I9X27_RS00635	139878..140264	+	387	WP_014602345.1	aegerolysin family protein	-
I9X27_RS00640	140296..141204	-	909	WP_016561475.1	LysR family transcriptional regulator	-
I9X27_RS00645	141504..141785	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
I9X27_RS00650	141782..142009	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
I9X27_RS00655	142185..142805	-	621	WP_003101226.1	hypothetical protein	-
I9X27_RS00660	142906..143406	+	501	WP_003112629.1	LEA type 2 family protein	-
I9X27_RS00665	143479..143820	+	342	WP_003101229.1	alkylphosphonate utilization protein	-
I9X27_RS00670	143902..145329	-	1428	WP_003083784.1	GABA permease	-
I9X27_RS00675	145498..146991	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T183565 WP_003083773.1 NZ_CP065866:c141785-141504 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T183565 NZ_CP087111:c4073022-4072735 [Pseudomonas palleroniana]
ATGATCTATAGCCTTGATTTTGATGCACGCGCATTGAAGGAATGGCAAAAACTCGGCAACACCATCCGTCAACAGCTCAA
GAAGAAGCTGATGGAAATTCTGAATGACCCACGCATTGAAGCTAACCGCCTGCATGGCCTGCCTGACTGCTACAAAATCA
AATTACGTAGCAGCGGTTATAGGCTTGTCTATCAAGTGATTGATCATGAAATCACTGTGTTCGTCGTGGCTGTTGATAAG
CGGGAACGTGATCGGGTTTATCAGAGAGCGGCTGAGCGTTTGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT183565 WP_003083775.1 NZ_CP065866:c142009-141782 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT183565 NZ_CP087111:c4073251-4073012 [Pseudomonas palleroniana]
ATGGCATCTATAAACATCCGCATCGATGACGACCTGAAAGCTCGCGCCTACCTGGAACTGGAAAGGCTTGGCGTCACGCC
TTCTGAACTGATGCGCCAAACCCTGCAATATGTAGTAGAGCGCGGTCAATTGCCTTTCAAGCCTGTGCTGATGACTGAGG
ATGATGAAGCCTTGCTGGCCAAAGTGCGGGAACGATTGGCGTCCCCTCAACGCGTAAGGGTTTCTATAGATGATCTATAG

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9