Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 5442029..5442637 Replicon chromosome
Accession NZ_CP065865
Organism Pseudomonas aeruginosa strain TJ2019-022

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag I9X26_RS25915 Protein ID WP_003114156.1
Coordinates 5442029..5442376 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag I9X26_RS25920 Protein ID WP_003114155.1
Coordinates 5442386..5442637 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I9X26_RS25885 5437387..5437659 + 273 WP_003120794.1 cysteine-rich CWC family protein -
I9X26_RS25890 5437659..5438351 + 693 WP_003098362.1 16S rRNA pseudouridine(516) synthase -
I9X26_RS25895 5438487..5439530 + 1044 WP_003098363.1 L,D-transpeptidase -
I9X26_RS25900 5439610..5440347 + 738 WP_003085453.1 murein L,D-transpeptidase catalytic domain family protein -
I9X26_RS25905 5440800..5441702 + 903 WP_003085447.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
I9X26_RS25915 5442029..5442376 - 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I9X26_RS25920 5442386..5442637 - 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I9X26_RS25925 5442851..5443834 - 984 WP_043154346.1 tyrosine-type recombinase/integrase -
I9X26_RS25930 5443834..5445126 - 1293 WP_003115206.1 hypothetical protein -
I9X26_RS25935 5445384..5446646 - 1263 WP_073655706.1 hypothetical protein -
I9X26_RS25940 5446648..5446998 - 351 WP_003159569.1 DUF2523 domain-containing protein -
I9X26_RS25945 5447008..5447589 - 582 WP_126870428.1 coat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 5442029..5461950 19921


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T183563 WP_003114156.1 NZ_CP065865:c5442376-5442029 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T183563 NZ_CP087111:c4024326-4024045 [Pseudomonas palleroniana]
ATGAGAGAACTCAAAGCATTTGATGCATCCCAGTACCTCACCAACGAGGACGAGATTGCTCAATTCCTGACCATTTCTTT
GGAAGACCCAAACCCTGACGTGTTCCTTCGCGCTCTGCAGGAAGTAGCCAAGGCTCGTGGCATGAGCCAGCTTGCCAAGG
ATGCTGGTATCAATCGTGAGAGCCTGTACAAAGCGTTGGCTCCAGGTGCAAAGCCCCGGTATGACACAGTGTTGAAGCTT
GTGAATGCATTAGGCGTGAAGCTGACGGCACAGCACGTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT183563 WP_003114155.1 NZ_CP065865:c5442637-5442386 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT183563 NZ_CP087111:c4024631-4024323 [Pseudomonas palleroniana]
ATGAACACAATCATCACTACCCATGAATTTGATAACTGGCTGAAAGGTCTGAAAGACGTACAGGCACGTCTACGGATCGT
TTCTCGGATCTTGAGTGCAGAGGCGGGTAATTTTGGTGACTGTGAGCCGGTGGGCGAAGGCATCAGCGAAATGCGGATCT
TCGTCGGGCCGGGATACCGTGTGTACTACACGCGCCGTGGCGAGGTCGTTTACCTGCTACTGTGTGGCGGCAATAAGTCA
TCACAGAAACGAGACATCAAGCTTGCCAAATCCATCCTCGATGAGATCGAGCAAGGAGACGTAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References