Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2176069..2176583 Replicon chromosome
Accession NZ_CP065857
Organism Staphylococcus aureus strain CC1153-MRSA

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag I1A60_RS10700 Protein ID WP_000581792.1
Coordinates 2176069..2176278 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag I1A60_RS10705 Protein ID WP_000138932.1
Coordinates 2176290..2176583 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I1A60_RS10680 2171577..2172935 - 1359 WP_000611466.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
I1A60_RS10685 2172950..2174020 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
I1A60_RS10690 2174338..2175540 + 1203 WP_001109939.1 rod shape-determining protein RodA -
I1A60_RS10695 2175804..2175941 - 138 WP_000828354.1 hypothetical protein -
I1A60_RS10700 2176069..2176278 - 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
I1A60_RS10705 2176290..2176583 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
I1A60_RS10710 2176749..2178233 + 1485 WP_000571549.1 cardiolipin synthase -
I1A60_RS10715 2178261..2178908 + 648 WP_001187630.1 HD domain-containing protein -
I1A60_RS10720 2179580..2180452 - 873 WP_000725802.1 membrane protein insertase YidC -
I1A60_RS10725 2180538..2181179 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T183547 WP_000581792.1 NZ_CP065857:c2176278-2176069 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T183547 NZ_CP087110:c2235899-2235723 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT183547 WP_000138932.1 NZ_CP065857:c2176583-2176290 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT183547 NZ_CP087110:c2235677-2235261 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References