Detailed information of TA system TA183542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1994242..1995018
• Replicon: chromosome
• Accession: NZ_CP065857
• Organism: Staphylococcus aureus strain CC1153-MRSA

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: I1A60_RS09635
• Protein ID: WP_000031108.1
• Coordinates: 1994242..1994394 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: -
• Locus tag: I1A60_RS09640
• Protein ID: WP_072468716.1
• Coordinates: 1994419..1995018 (-)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I1A60_RS09620	1990319..1991140	+	822	WP_000669376.1	RluA family pseudouridine synthase	-
I1A60_RS09625	1991603..1992988	-	1386	WP_000116236.1	class II fumarate hydratase	-
I1A60_RS09630	1993184..1993579	-	396	WP_000901023.1	hypothetical protein	-
I1A60_RS09635	1994242..1994394	-	153	WP_000031108.1	hypothetical protein	Toxin
I1A60_RS09640	1994419..1995018	-	600	WP_072468716.1	glucosamine-6-phosphate isomerase	Antitoxin
I1A60_RS09645	1995177..1995647	-	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
I1A60_RS09650	1995644..1996780	-	1137	WP_198051135.1	tRNA epoxyqueuosine(34) reductase QueG	-
I1A60_RS09655	1996931..1997653	-	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
I1A60_RS09660	1997646..1999103	-	1458	WP_000649908.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T183542 WP_000031108.1 NZ_CP065857:c1994394-1994242 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T183542 NZ_CP087110:1653822-1654076 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22342.53 Da
• Isoelectric Point: 5.1445

>AT183542 WP_072468716.1 NZ_CP065857:c1995018-1994419 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDLAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNIILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT183542 NZ_CP087110:1653574-1653825 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: X5E2E6