Detailed information of TA system TA183537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 5200228..5200879
• Replicon: chromosome
• Accession: NZ_CP065848
• Organism: Pseudomonas aeruginosa strain CMC-097

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IAU57_RS24300
• Protein ID: WP_019486655.1
• Coordinates: 5200228..5200407 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: IAU57_RS24305
• Protein ID: WP_019486656.1
• Coordinates: 5200448..5200879 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU57_RS24270	5195845..5197200	-	1356	WP_016263341.1	DUF4055 domain-containing protein	-
IAU57_RS24275	5197203..5198528	-	1326	WP_088170146.1	terminase family protein	-
IAU57_RS24280	5198600..5199106	-	507	Protein_4795	terminase small subunit	-
IAU57_RS24285	5199115..5199399	-	285	WP_010793139.1	phage holin family protein	-
IAU57_RS24290	5199392..5199781	-	390	WP_019486653.1	hypothetical protein	-
IAU57_RS24295	5199924..5200139	-	216	WP_019486654.1	hypothetical protein	-
IAU57_RS24300	5200228..5200407	+	180	WP_019486655.1	type II toxin-antitoxin system HicA family toxin	Toxin
IAU57_RS24305	5200448..5200879	+	432	WP_019486656.1	transcriptional regulator	Antitoxin
IAU57_RS24310	5200969..5201856	-	888	WP_198128990.1	hypothetical protein	-
IAU57_RS24315	5201979..5202425	-	447	WP_019486658.1	RusA family crossover junction endodeoxyribonuclease	-
IAU57_RS24320	5202422..5202724	-	303	WP_019486659.1	hypothetical protein	-
IAU57_RS24325	5202717..5203127	-	411	WP_025991903.1	hypothetical protein	-
IAU57_RS24330	5203255..5204217	-	963	WP_031652668.1	hypothetical protein	-
IAU57_RS24335	5204218..5204533	-	316	Protein_4806	transcriptional regulator	-
IAU57_RS24340	5204536..5204739	-	204	WP_031692832.1	helix-turn-helix domain-containing protein	-
IAU57_RS24345	5204822..5205517	+	696	WP_171946357.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5172519..5240092	67573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-78)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6605.65 Da
• Isoelectric Point: 11.1475

>T183537 WP_019486655.1 NZ_CP065848:5200228-5200407 [Pseudomonas aeruginosa]
MKFSEFRRWLRAQGVTFEAGKGSHFKVTAPNGKTTTFADHGAKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T183537 NZ_CP087110:699916-700323 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15862.17 Da
• Isoelectric Point: 4.7692

>AT183537 WP_019486656.1 NZ_CP065848:5200448-5200879 [Pseudomonas aeruginosa]
MYNFAIRFEQDDSAPGVAVFCRDLPELNSYGNTVEHALGEAVDAIETTLSLYVDQRRPIPAASAPEPEEHVVHLPAVTVA
KIALWNEMMRRDMRKADLCRALGVAQVQVDRLVDFLHTSKMEQLESALGVLGLRLFVSVQQAA

Nucleotide

• Length: 432 bp

>AT183537 NZ_CP087110:699669-699935 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA