Detailed information of TA system TA183536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5068534..5069168
• Replicon: chromosome
• Accession: NZ_CP065848
• Organism: Pseudomonas aeruginosa strain CMC-097

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IAU57_RS23540
• Protein ID: WP_003291451.1
• Coordinates: 5068534..5068938 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: IAU57_RS23545
• Protein ID: WP_003285520.1
• Coordinates: 5068938..5069168 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU57_RS23520	5064731..5066140	+	1410	WP_126550986.1	site-specific integrase	-
IAU57_RS23525	5066134..5066427	-	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IAU57_RS23530	5066415..5066687	-	273	WP_116801393.1	CopG family ribbon-helix-helix protein	-
IAU57_RS23535	5067482..5068450	+	969	WP_126550988.1	DUF932 domain-containing protein	-
IAU57_RS23540	5068534..5068938	-	405	WP_003291451.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IAU57_RS23545	5068938..5069168	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IAU57_RS23550	5069325..5070332	+	1008	WP_126550994.1	YqaJ viral recombinase family protein	-
IAU57_RS23555	5070422..5071366	+	945	WP_126550990.1	hydrolase or metal-binding protein	-
IAU57_RS23560	5071338..5071715	+	378	Protein_4653	DNA repair protein RadC	-
IAU57_RS23565	5071880..5073394	+	1515	WP_011914447.1	IS21 family transposase	-
IAU57_RS23570	5073412..5074167	+	756	WP_011914448.1	IS21-like element helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5064848..5118451	53603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14968.21 Da
• Isoelectric Point: 7.2774

>T183536 WP_003291451.1 NZ_CP065848:c5068938-5068534 [Pseudomonas aeruginosa]
MLKYMLDTNMCIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVSA

Nucleotide

• Length: 405 bp

>T183536 NZ_CP087110:572195-572491 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT183536 WP_003285520.1 NZ_CP065848:c5069168-5068938 [Pseudomonas aeruginosa]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT183536 NZ_CP087110:572493-572888 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: L1LS63