Detailed information of TA system TA183535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 5066134..5066687
• Replicon: chromosome
• Accession: NZ_CP065848
• Organism: Pseudomonas aeruginosa strain CMC-097

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q88JZ3
• Locus tag: IAU57_RS23525
• Protein ID: WP_010953434.1
• Coordinates: 5066134..5066427 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IAU57_RS23530
• Protein ID: WP_116801393.1
• Coordinates: 5066415..5066687 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU57_RS23505	5061583..5061804	+	222	WP_023088234.1	SymE family type I addiction module toxin	-
IAU57_RS23510	5062197..5062577	+	381	WP_003155927.1	nucleotide pyrophosphohydrolase	-
IAU57_RS23515	5062605..5064461	+	1857	WP_023088233.1	DUF2075 domain-containing protein	-
IAU57_RS23520	5064731..5066140	+	1410	WP_126550986.1	site-specific integrase	-
IAU57_RS23525	5066134..5066427	-	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAU57_RS23530	5066415..5066687	-	273	WP_116801393.1	CopG family ribbon-helix-helix protein	Antitoxin
IAU57_RS23535	5067482..5068450	+	969	WP_126550988.1	DUF932 domain-containing protein	-
IAU57_RS23540	5068534..5068938	-	405	WP_003291451.1	tRNA(fMet)-specific endonuclease VapC	-
IAU57_RS23545	5068938..5069168	-	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
IAU57_RS23550	5069325..5070332	+	1008	WP_126550994.1	YqaJ viral recombinase family protein	-
IAU57_RS23555	5070422..5071366	+	945	WP_126550990.1	hydrolase or metal-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5064848..5118451	53603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11155.61 Da
• Isoelectric Point: 5.9081

>T183535 WP_010953434.1 NZ_CP065848:c5066427-5066134 [Pseudomonas aeruginosa]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T183535 NZ_CP087110:506284-506598 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10523.65 Da
• Isoelectric Point: 5.6460

>AT183535 WP_116801393.1 NZ_CP065848:c5066687-5066415 [Pseudomonas aeruginosa]
MATSIKIDDELKGRIQQLASQRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGRHLTSQETRTWLGSWGTD
TEVELPKCHD

Nucleotide

• Length: 273 bp

>AT183535 NZ_CP087110:506595-507011 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A2W5CNE8