Detailed information of TA system TA183532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 2754535..2755793
• Replicon: chromosome
• Accession: NZ_CP065848
• Organism: Pseudomonas aeruginosa strain CMC-097

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: IAU57_RS12935
• Protein ID: WP_023084953.1
• Coordinates: 2755038..2755793 (+)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: IAU57_RS12930
• Protein ID: WP_003086329.1
• Coordinates: 2754535..2754903 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU57_RS12910	2750698..2750973	+	276	WP_171945305.1	zinc ribbon domain-containing protein	-
IAU57_RS12915	2750976..2751452	-	477	WP_023122241.1	hypothetical protein	-
IAU57_RS12920	2751531..2753120	+	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
IAU57_RS12925	2753176..2754462	+	1287	WP_087851268.1	FAD-binding oxidoreductase	-
IAU57_RS12930	2754535..2754903	+	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
IAU57_RS12935	2755038..2755793	+	756	WP_023084953.1	RES family NAD+ phosphorylase	Toxin
IAU57_RS12940	2756561..2757922	+	1362	WP_003081943.1	DNA recombination protein RmuC	-
IAU57_RS12945	2758077..2760620	-	2544	WP_126550779.1	acyl-homoserine-lactone acylase QuiP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28310.49 Da
• Isoelectric Point: 7.7294

>T183532 WP_023084953.1 NZ_CP065848:2755038-2755793 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGMRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T183532 NZ_CP087106:1251093-1251494 [Methylobacterium sp. NMS14P]
ATGTTCGTCGACGCGTCCGCCATCGTGGCGATCGTCACCGGCGAGCCAGGACATCGTACGCTGCTGGATCGGTTGCGCGA
CGGTTCCGTGCGGCTCACGAGCGGCGTCGCCGTCTACGAGGCGGTGCTCGCCATCGCTTGGCTCCAGGCCGTCCCGCCCG
AGGTCGCTCGAGAGATCGTGGACGATTTCCTGGACGCCGCCGGCGTCACACGGGTCCCGATCGCCGAAGCGGAGGCGCGC
CTCGCCGTCATGGCCCACGCCCGCTACGGCAAGGGCCGCCACCCGGCGCGCCTGAACCTCGGCGACTGCTTCGCCTATGC
CTTCGCGCGGGTGCACGGCGTGCCGCTGCTCTACGTCGGCGACGAATTCCCGCAGACCGACATCCGCTCGGCCCTCGGCT
GA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT183532 WP_003086329.1 NZ_CP065848:2754535-2754903 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT183532 NZ_CP087106:1250854-1251093 [Methylobacterium sp. NMS14P]
ATGCCGCTCAACATCCGCAGCGAAGAGGTGAATCAGCTGGCCGAGAAGCTGGCATCGCGGGCCGGCGTGAGCAAGACCGA
GGCGGTGCGACTCGCGCTCGCGAACGAGCTGGCGCGGCGGGACGCGGAGCGCGAGACCTTCCTGGAGAGGGTCACGCAGA
TCCAGGAAAGGCTGGCGTCCTACCCGTCAACGGGCCTCCAGGCAGACAAGGCGTTCTACGACAGCCTGAACGACGAGTGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7