Detailed information of TA system TA183531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 2021966..2022552
• Replicon: chromosome
• Accession: NZ_CP065848
• Organism: Pseudomonas aeruginosa strain CMC-097

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: IAU57_RS09500
• Protein ID: WP_003120987.1
• Coordinates: 2021966..2022265 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IAU57_RS09505
• Protein ID: WP_003448662.1
• Coordinates: 2022262..2022552 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IAU57_RS09480	2017079..2017612	-	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-
IAU57_RS09485	2017602..2018927	-	1326	WP_024947295.1	type 4b pilus protein PilO2	-
IAU57_RS09490	2018931..2020640	-	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
IAU57_RS09495	2020640..2021764	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
IAU57_RS09500	2021966..2022265	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IAU57_RS09505	2022262..2022552	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
IAU57_RS09510	2022623..2022967	-	345	WP_016851612.1	hypothetical protein	-
IAU57_RS09515	2023108..2025084	-	1977	WP_016851611.1	DEAD/DEAH box helicase	-
IAU57_RS09520	2025081..2026970	-	1890	WP_016851610.1	hypothetical protein	-
IAU57_RS09525	2027192..2027401	-	210	WP_003105733.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul1 / blaOXA-2	-	1931888..2052135	120247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T183531 WP_003120987.1 NZ_CP065848:2021966-2022265 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T183531 NZ_CP087103:2324327-2325067 [Bacillus toyonensis]
TTGACGATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTTTTTGTAGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTATATGAAGAGAAAAAGCAGCGAATTCGAAAAACTTGGTATGGATTATTCATAACATCAGTCACAGTTTATT
TCATGATTAAAGGAATCGATTTAACCCTCTGGAAAAACTTATTAATGTTCACTGCGATGGTCATTTTTGTTGATATTTCA
TTTATTTTAACACCTAATATTTCCGAAATATGGGGAGCGAAATTTAGTGATATCGGTAAAACTGTTCAATCCATAAAGCG
GTCATTAATCGCTTCAAAAGCAAGAGGAGAAATATATACGAACATTATCCAAAATATTAATCCAGCAGCATTTGGGACGA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAAATTGGAGCGAAA
ATTGTTGTATTTGAAGCAGCGAATGAATTAAATACGGGCTTTCGTGGTATTCGTTCTCAATTTAGTATTACTATACCTAT
AGAGCATATAGAGCAATTGAATGAACAAAAAGCAGTGCAAGTAGAAAACGTGGGAATTATACCAGCAAAAATAATGAATG
ATGTTTTTATCGTGATTGATGGGAAGAAGAATAGCCTGCAGGATCGAGATTTTGAAAATGTATATAATTTAACGATACAT
CATAGTTATTTTAGTAAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT183531 WP_003448662.1 NZ_CP065848:2022262-2022552 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT183531 NZ_CP087103:2325180-2325305 [Bacillus toyonensis]
GTGATAAAAGATTATGACAATGATTTTTTCAAAGAAGAAAATGAAGATACAACAGATTTCTCTTTAATCAAAGGAGATAC
GTTGCAGCAAGTAGAAAAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: G8CP73