Detailed information of TA system TA183527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 88006..88742
• Replicon: plasmid p1
• Accession: NZ_CP065847
• Organism: Klebsiella quasipneumoniae strain KqPF42

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I8N77_RS26040
• Protein ID: WP_003026803.1
• Coordinates: 88260..88742 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I8N77_RS26035
• Protein ID: WP_003026799.1
• Coordinates: 88006..88272 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N77_RS26000	83198..83548	-	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I8N77_RS26005	83906..84154	+	249	WP_004193994.1	hypothetical protein	-
I8N77_RS26010	84151..85362	+	1212	WP_032415728.1	hypothetical protein	-
I8N77_RS26015	85496..85987	+	492	WP_004206662.1	hypothetical protein	-
I8N77_RS26020	86139..87107	-	969	WP_022652343.1	IS5-like element IS903B family transposase	-
I8N77_RS26025	87087..87317	+	231	WP_199489677.1	hemolysin expression modulator Hha	-
I8N77_RS26030	87331..87561	+	231	WP_004206660.1	hypothetical protein	-
I8N77_RS26035	88006..88272	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I8N77_RS26040	88260..88742	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I8N77_RS26045	88943..90346	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
I8N77_RS26050	90375..91007	-	633	WP_001567369.1	hypothetical protein	-
I8N77_RS26055	91236..92582	+	1347	WP_077251107.1	ISNCY family transposase	-
I8N77_RS26060	92742..93446	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..223863	223863
	-	inside	IScluster/Tn	-	-	86139..95837	9698

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T183527 WP_003026803.1 NZ_CP065847:88260-88742 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T183527 NZ_CP087094:12216-12611 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT183527 WP_003026799.1 NZ_CP065847:88006-88272 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT183527 NZ_CP087094:11999-12226 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66