Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 32098..32633 Replicon plasmid p1
Accession NZ_CP065847
Organism Klebsiella quasipneumoniae strain KqPF42

Toxin (Protein)


Gene name relE Uniprot ID A0A2A5MBV5
Locus tag I8N77_RS25700 Protein ID WP_032420954.1
Coordinates 32098..32385 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2A5MCI9
Locus tag I8N77_RS25705 Protein ID WP_032420955.1
Coordinates 32382..32633 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N77_RS25670 27232..28206 + 975 WP_032752080.1 StbA family protein -
I8N77_RS25675 28210..28602 + 393 WP_032420949.1 plasmid partitioning/stability family protein -
I8N77_RS25680 29252..30376 + 1125 WP_096834042.1 hypothetical protein -
I8N77_RS25685 30595..31341 + 747 WP_032420989.1 hypothetical protein -
I8N77_RS25690 31643..31921 - 279 WP_032420952.1 hypothetical protein -
I8N77_RS25695 31942..32091 - 150 WP_158238164.1 hypothetical protein -
I8N77_RS25700 32098..32385 - 288 WP_032420954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I8N77_RS25705 32382..32633 - 252 WP_032420955.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I8N77_RS25710 32800..34071 - 1272 WP_032420957.1 Y-family DNA polymerase -
I8N77_RS25715 34071..34496 - 426 WP_001776124.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
I8N77_RS25720 34899..36410 + 1512 WP_024623221.1 group II intron reverse transcriptase/maturase -
I8N77_RS25725 36644..36874 + 231 WP_004206779.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..223863 223863


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.13 Da        Isoelectric Point: 10.2246

>T183526 WP_032420954.1 NZ_CP065847:c32385-32098 [Klebsiella quasipneumoniae]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDHLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T183526 NZ_CP087093:c181942-181616 [Pseudosulfitobacter pseudonitzschiae]
GTGAGGCGTGGTGAGCTGGTGACGGTCGCCCTTCAGGGCGACCACGGCAAGCCGCGTCCCGCGCTTGTGATCCAATCCGA
CCGGTTTCCGGACACCGCCACCACGACCGTCCTTCTGGTTACATCCACCTTGGTGGATGCGCCGTTGATTCGGCTGACGG
TCGAACCCTCTGCGGAGAACGGGTTGCGTATGCGATCACAGATCATGATCGACAAAGCCGTGACAGTTCGAACGGACAAG
CTGGGGAAAGCTTTCGGTCAGCTGGACGATACCGCCATGATCGAGGTGAACCGGGCACTAGCACTGTTTTTAGGAGTGGC
GGGGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9192.47 Da        Isoelectric Point: 3.9507

>AT183526 WP_032420955.1 NZ_CP065847:c32633-32382 [Klebsiella quasipneumoniae]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDAMDDQELAKLVSERSNQQLLDVDLD
TYL

Download         Length: 252 bp

>AT183526 NZ_CP087093:c182157-181939 [Pseudosulfitobacter pseudonitzschiae]
ATGCCAACCTCTGTTTCTCACCGTGTCCAGAAGCGTCGCGATGCCTTGCGTGCCGCTGGTTTGCGCCCCGTCCAGATTTG
GGTGCCGGATACCCGCCGCCCCGGTTTCGAGGCTGAGTGCCGCCGCCAGGCTGCAATTGTAGCCAAGGCCGATCGCGCAG
ATGATACTCTGAACGATTTCCTGGATGCCTCCCTGGTCGATGTGGATGACTGGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBV5

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MCI9

References