Detailed information of TA system TA183523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2679091..2680679
• Replicon: chromosome
• Accession: NZ_CP065846
• Organism: Klebsiella quasipneumoniae strain KqPF42

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: I8N77_RS13125
• Protein ID: WP_048324183.1
• Coordinates: 2679091..2680413 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: I8N77_RS13130
• Protein ID: WP_004205921.1
• Coordinates: 2680413..2680679 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N77_RS13090	2674173..2674391	+	219	WP_002904808.1	ParD-like family protein	-
I8N77_RS13095	2674372..2675154	+	783	WP_048294851.1	type I methionyl aminopeptidase	-
I8N77_RS13100	2675423..2676538	+	1116	WP_048324188.1	serine hydrolase	-
I8N77_RS13105	2676525..2676869	+	345	WP_004205915.1	hypothetical protein	-
I8N77_RS13110	2676898..2677431	+	534	WP_004205916.1	hypothetical protein	-
I8N77_RS13115	2677434..2677640	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
I8N77_RS13120	2677782..2679074	+	1293	WP_048324186.1	glycoside hydrolase family 10 protein	-
I8N77_RS13125	2679091..2680413	-	1323	WP_048324183.1	type II toxin-antitoxin system HipA family toxin	Toxin
I8N77_RS13130	2680413..2680679	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I8N77_RS13135	2680962..2681288	-	327	WP_017900379.1	hydroxyisourate hydrolase	-
I8N77_RS13140	2681285..2681785	-	501	WP_048324181.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
I8N77_RS13145	2681763..2683142	-	1380	WP_048324178.1	purine permease	-
I8N77_RS13150	2683488..2684642	+	1155	WP_032456029.1	FAD-dependent urate hydroxylase HpxO	-
I8N77_RS13155	2684623..2685546	-	924	WP_048324174.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	2489063..2737079	248016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 48899.16 Da
• Isoelectric Point: 7.8920

>T183523 WP_048324183.1 NZ_CP065846:c2680413-2679091 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRVP
QAIESARQTLPSDFSAHVWGAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T183523 NZ_CP087091:104217-104468 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT183523 WP_004205921.1 NZ_CP065846:c2680679-2680413 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT183523 NZ_CP087091:104465-104731 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9