Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 289866..290509 Replicon plasmid p1
Accession NZ_CP065842
Organism Klebsiella quasipneumoniae strain KqPF9

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag I8N79_RS27145 Protein ID WP_001044770.1
Coordinates 289866..290282 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag I8N79_RS27150 Protein ID WP_001261282.1
Coordinates 290279..290509 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N79_RS27125 285235..285585 + 351 WP_004187110.1 DUF305 domain-containing protein -
I8N79_RS27130 285771..286679 - 909 WP_032425603.1 HNH endonuclease -
I8N79_RS27135 287223..288245 - 1023 WP_000361404.1 helicase UvrD -
I8N79_RS27140 288230..289792 - 1563 WP_009309907.1 AAA family ATPase -
I8N79_RS27145 289866..290282 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
I8N79_RS27150 290279..290509 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
I8N79_RS27155 290466..290702 + 237 Protein_305 hypothetical protein -
I8N79_RS27160 290801..291394 + 594 WP_009309909.1 hypothetical protein -
I8N79_RS27165 291413..291853 + 441 WP_047057603.1 hypothetical protein -
I8N79_RS27170 292044..292980 - 937 Protein_308 CAAX protease -
I8N79_RS27175 293302..293646 + 345 WP_009309911.1 hypothetical protein -
I8N79_RS27180 293754..294389 - 636 WP_009309912.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..399394 399394


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T183513 WP_001044770.1 NZ_CP065842:c290282-289866 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T183513 NZ_CP087083:1906072-1906335 [Pseudosulfitobacter pseudonitzschiae]
ATGAAGTTCGAGTATGACCCTGACAAGAGTGCCGGGAACAAGGCCAAGCACGGCATCGACTTCGATGAAGCGCAAGCGCT
TTGGGAAGACCCGTGGCTGTTGGAGGCTCCCGCTCGAACAGATGACGAGCCGCGCTTTTTTGCAATAGGCATGATCAAGG
AACGGCATTGGTCAGCAGTTTGCACCTATCGCGGTGATATTATTCGGATCATCTCGGTGCGCCGCGCCAGAAAACAGGAG
ATTGAGCACTATGAAAGCACGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT183513 WP_001261282.1 NZ_CP065842:c290509-290279 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT183513 NZ_CP087083:1906322-1906537 [Pseudosulfitobacter pseudonitzschiae]
ATGAAAGCACGTGAGTTCGATCAAAAATTTGACACAGGTGAGAACGTCAGCGATCTTGTGGACTGGTCCAAAGCTAGGCG
GACGAATACCCAGACGAAGCGAGTCAATGTGGATTTTCCGTCGTGGGTCGTTTCTGGTTTGGATCAGAAGGCAAAGCAGC
TCGGCGTTACTCGTCAGTCGCTCATCAAACTCTGGATCGCCGAGCGGCTCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References