Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 183537..184447 Replicon plasmid p1
Accession NZ_CP065842
Organism Klebsiella quasipneumoniae strain KqPF9

Toxin (Protein)


Gene name - Uniprot ID A0A2N4Z2R0
Locus tag I8N79_RS26540 Protein ID WP_004197882.1
Coordinates 183537..184007 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag I8N79_RS26545 Protein ID WP_004181895.1
Coordinates 184004..184447 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N79_RS26525 179751..181283 - 1533 WP_040088343.1 IS3 family transposase -
I8N79_RS26530 181424..182605 + 1182 WP_061891937.1 IS481 family transposase -
I8N79_RS26535 183167..183427 + 261 WP_004026352.1 hypothetical protein -
I8N79_RS26540 183537..184007 - 471 WP_004197882.1 RES family NAD+ phosphorylase Toxin
I8N79_RS26545 184004..184447 - 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
I8N79_RS26550 184548..185723 - 1176 Protein_184 translesion error-prone DNA polymerase V subunit UmuC -
I8N79_RS26555 185866..186324 + 459 Protein_185 recombinase family protein -
I8N79_RS26560 186575..186784 + 210 WP_072072310.1 microcin B17 -
I8N79_RS26565 186843..187697 + 855 WP_048268726.1 McbB family protein -
I8N79_RS26570 187699..188505 + 807 WP_048268727.1 SagB/ThcOx family dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..399394 399394
- inside IScluster/Tn - - 179751..186324 6573


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17633.92 Da        Isoelectric Point: 4.5152

>T183512 WP_004197882.1 NZ_CP065842:c184007-183537 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDFFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T183512 NZ_CP087081:6992-7258 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT183512 WP_004181895.1 NZ_CP065842:c184447-184004 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT183512 NZ_CP087081:6744-6995 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N4Z2R0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References