Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 236106..236631 Replicon chromosome
Accession NZ_CP065841
Organism Klebsiella quasipneumoniae strain KqPF9

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag I8N79_RS01145 Protein ID WP_001568026.1
Coordinates 236326..236631 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag I8N79_RS01140 Protein ID WP_001568025.1
Coordinates 236106..236324 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N79_RS01110 231743..232267 - 525 Protein_220 IS5-like element ISKpn26 family transposase -
I8N79_RS01115 232303..233742 - 1440 Protein_221 IS66 family transposase -
I8N79_RS01120 233807..234511 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
I8N79_RS01125 234597..234737 + 141 WP_045286941.1 helix-turn-helix domain-containing protein -
I8N79_RS01130 234835..235251 - 417 WP_021312768.1 type II toxin-antitoxin system VapC family toxin -
I8N79_RS01135 235248..235478 - 231 WP_021312767.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
I8N79_RS01140 236106..236324 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I8N79_RS01145 236326..236631 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
I8N79_RS01150 236800..237195 + 396 WP_017899885.1 hypothetical protein -
I8N79_RS01155 237222..237545 + 324 WP_022644730.1 hypothetical protein -
I8N79_RS01160 237542..238558 + 1017 WP_020315256.1 hypothetical protein -
I8N79_RS01165 238756..239550 + 795 WP_004197635.1 site-specific integrase -
I8N79_RS01170 240006..240185 - 180 WP_006788217.1 hypothetical protein -
I8N79_RS01175 240305..240931 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T183497 WP_001568026.1 NZ_CP065841:236326-236631 [Klebsiella quasipneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T183497 NZ_CP087067:c98542-98147 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT183497 WP_001568025.1 NZ_CP065841:236106-236324 [Klebsiella quasipneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT183497 NZ_CP087067:c98759-98532 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References