Detailed information of TA system TA183493

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4631511..4632202
• Replicon: chromosome
• Accession: NZ_CP065838
• Organism: Klebsiella quasipneumoniae strain KqPF26

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A483YFJ1
• Locus tag: I8N75_RS22465
• Protein ID: WP_025987721.1
• Coordinates: 4631511..4631852 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: I8N75_RS22470
• Protein ID: WP_038434619.1
• Coordinates: 4631876..4632202 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N75_RS22450	4628525..4629760	-	1236	WP_198974684.1	hypothetical protein	-
I8N75_RS22455	4630165..4630497	-	333	WP_074439534.1	hypothetical protein	-
I8N75_RS22460	4630564..4631397	-	834	WP_198974766.1	DUF4942 domain-containing protein	-
I8N75_RS22465	4631511..4631852	-	342	WP_025987721.1	TA system toxin CbtA family protein	Toxin
I8N75_RS22470	4631876..4632202	-	327	WP_038434619.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I8N75_RS22475	4632217..4632693	-	477	WP_038434581.1	DNA repair protein RadC	-
I8N75_RS22480	4632704..4633150	-	447	WP_038434582.1	antirestriction protein	-
I8N75_RS22485	4633374..4634063	-	690	WP_038434583.1	hypothetical protein	-
I8N75_RS22490	4634383..4634736	+	354	WP_038434584.1	hypothetical protein	-
I8N75_RS22495	4634773..4635663	-	891	WP_198974685.1	50S ribosome-binding GTPase	-
I8N75_RS22500	4635735..4636823	-	1089	WP_152651583.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4628525..4647774	19249

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12867.90 Da
• Isoelectric Point: 8.5195

>T183493 WP_025987721.1 NZ_CP065838:c4631852-4631511 [Klebsiella quasipneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Nucleotide

• Length: 342 bp

>T183493 NZ_CP087063:227999-228265 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12304.06 Da
• Isoelectric Point: 6.9560

>AT183493 WP_038434619.1 NZ_CP065838:c4632202-4631876 [Klebsiella quasipneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDVECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT183493 NZ_CP087063:227751-228002 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A483YFJ1