Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 291654..292564 Replicon plasmid p1
Accession NZ_CP065834
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag I8N78_RS26375 Protein ID WP_004144067.1
Coordinates 291654..292124 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag I8N78_RS26380 Protein ID WP_004026357.1
Coordinates 292121..292564 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS26340 286965..287231 - 267 WP_003026799.1 DUF1778 domain-containing protein -
I8N78_RS26345 287364..287792 - 429 WP_004901287.1 GFA family protein -
I8N78_RS26350 287993..288139 - 147 WP_159193595.1 IS3 family transposase -
I8N78_RS26355 288161..289281 + 1121 WP_085955508.1 IS3 family transposase -
I8N78_RS26360 289308..290123 - 816 WP_077268817.1 IS3 family transposase -
I8N78_RS26365 290078..290587 - 510 WP_004901294.1 IS3 family transposase -
I8N78_RS26370 291283..291543 + 261 WP_048291871.1 hypothetical protein -
I8N78_RS26375 291654..292124 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
I8N78_RS26380 292121..292564 - 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
I8N78_RS26385 292665..293171 - 507 Protein_282 DUF4113 domain-containing protein -
I8N78_RS26390 293272..293493 - 222 WP_072060588.1 DUF2188 domain-containing protein -
I8N78_RS26395 293917..294654 + 738 Protein_284 DUF1311 domain-containing protein -
I8N78_RS26400 294654..294995 + 342 WP_048291869.1 hypothetical protein -
I8N78_RS26405 295390..296435 + 1046 Protein_286 IS3 family transposase -
I8N78_RS26410 296490..296864 + 375 WP_173675457.1 hypothetical protein -
I8N78_RS26415 296794..297018 + 225 WP_127807699.1 DUF4113 domain-containing protein -
I8N78_RS26420 297097..297325 + 229 Protein_289 type II toxin-antitoxin system Phd/YefM family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS 1..301560 301560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T183480 WP_004144067.1 NZ_CP065834:c292124-291654 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T183480 NZ_CP065834:c292124-291654 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTAATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT183480 WP_004026357.1 NZ_CP065834:c292564-292121 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT183480 NZ_CP065834:c292564-292121 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTCCTGGGGCAAATCAATGAAGGCCTGGACGGGAAGGTGGCAAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCTTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGTATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAATGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACACGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References