Detailed information of TA system TA183479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 286492..287231
• Replicon: plasmid p1
• Accession: NZ_CP065834
• Organism: Klebsiella pneumoniae strain KpC5

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: I8N78_RS26335
• Protein ID: WP_021312536.1
• Coordinates: 286492..286977 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I8N78_RS26340
• Protein ID: WP_003026799.1
• Coordinates: 286965..287231 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N78_RS26305	281636..282073	-	438	WP_126034224.1	hypothetical protein	-
I8N78_RS26310	282169..283236	-	1068	WP_048291875.1	hypothetical protein	-
I8N78_RS26315	283305..283601	-	297	WP_048291874.1	hypothetical protein	-
I8N78_RS26320	283835..284212	+	378	WP_032719541.1	hypothetical protein	-
I8N78_RS26325	284409..285383	-	975	WP_180297170.1	Hok/Gef family protein	-
I8N78_RS26330	285988..286149	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
I8N78_RS26335	286492..286977	-	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
I8N78_RS26340	286965..287231	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I8N78_RS26345	287364..287792	-	429	WP_004901287.1	GFA family protein	-
I8N78_RS26350	287993..288139	-	147	WP_159193595.1	IS3 family transposase	-
I8N78_RS26355	288161..289281	+	1121	WP_085955508.1	IS3 family transposase	-
I8N78_RS26360	289308..290123	-	816	WP_077268817.1	IS3 family transposase	-
I8N78_RS26365	290078..290587	-	510	WP_004901294.1	IS3 family transposase	-
I8N78_RS26370	291283..291543	+	261	WP_048291871.1	hypothetical protein	-
I8N78_RS26375	291654..292124	-	471	WP_004144067.1	RES family NAD+ phosphorylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS	1..301560	301560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T183479 WP_021312536.1 NZ_CP065834:c286977-286492 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T183479 NZ_CP087050:3072-3347 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT183479 WP_003026799.1 NZ_CP065834:c287231-286965 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT183479 NZ_CP087050:2786-3079 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66