Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 249347..249872 Replicon plasmid p1
Accession NZ_CP065834
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag I8N78_RS26155 Protein ID WP_004197633.1
Coordinates 249567..249872 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID R4WC25
Locus tag I8N78_RS26150 Protein ID WP_015632547.1
Coordinates 249347..249565 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS26125 244773..245357 - 585 WP_023345591.1 TetR/AcrR family transcriptional regulator -
I8N78_RS26130 245469..245864 - 396 WP_023345592.1 helix-turn-helix transcriptional regulator -
I8N78_RS26135 245967..246812 + 846 WP_023345593.1 SDR family oxidoreductase -
I8N78_RS26140 246925..247353 + 429 WP_023345594.1 nuclear transport factor 2 family protein -
I8N78_RS26145 247695..249068 - 1374 WP_048265005.1 hypothetical protein -
I8N78_RS26150 249347..249565 + 219 WP_015632547.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I8N78_RS26155 249567..249872 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
I8N78_RS26160 250059..251047 + 989 Protein_237 hypothetical protein -
I8N78_RS26165 251246..252031 + 786 WP_048291886.1 site-specific integrase -
I8N78_RS26170 252353..252658 - 306 WP_126034233.1 hypothetical protein -
I8N78_RS26175 252807..253184 - 378 WP_158672279.1 DUF1173 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS 1..301560 301560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T183478 WP_004197633.1 NZ_CP065834:249567-249872 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T183478 NZ_CP087047:78982-79377 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7247

>AT183478 WP_015632547.1 NZ_CP065834:249347-249565 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Download         Length: 219 bp

>AT183478 NZ_CP087047:78765-78992 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1KT86

References