Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 228286..229090 Replicon plasmid p1
Accession NZ_CP065834
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name KacT Uniprot ID -
Locus tag I8N78_RS26045 Protein ID WP_072198182.1
Coordinates 228563..229090 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag I8N78_RS26040 Protein ID WP_048264996.1
Coordinates 228286..228555 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS26030 224939..226186 - 1248 WP_048264960.1 nucleotidyltransferase -
I8N78_RS26035 226183..227358 - 1176 WP_077258344.1 SAVED domain-containing protein -
I8N78_RS26040 228286..228555 + 270 WP_048264996.1 DUF1778 domain-containing protein Antitoxin
I8N78_RS26045 228563..229090 + 528 WP_072198182.1 GNAT family N-acetyltransferase Toxin
I8N78_RS26050 229174..229482 + 309 WP_048291890.1 hypothetical protein -
I8N78_RS26055 229533..230230 + 698 Protein_216 IS1 family transposase -
I8N78_RS26060 230542..231444 - 903 WP_048265000.1 LysR family transcriptional regulator -
I8N78_RS26065 231542..232150 + 609 WP_023345578.1 short chain dehydrogenase -
I8N78_RS26070 232269..233450 - 1182 WP_023345579.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS 1..301560 301560
- flank IS/Tn - - 229727..230230 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(54-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 20078.04 Da        Isoelectric Point: 5.7389

>T183476 WP_072198182.1 NZ_CP065834:228563-229090 [Klebsiella pneumoniae]
VMQELAIEMINDEFDYDIGEFDCGEETLNTFLKDHLKRQHDGQILKGYVLVTKEQKPKLLGYYTLSGSCFERKMLPSKTQ
QRKIPYQNAPSITLGRLAIDKSIQGQGWGELLVTHAMNVVWDASKAVGIYGLFVEALNDKAKRFYTRLDFIQLVGENSNL
LFYPTKSIEKIFTEE

Download         Length: 528 bp

>T183476 NZ_CP087044:170971-171297 [Pseudosulfitobacter pseudonitzschiae]
GTGAGGCGTGGTGAGCTGGTGACGGTCGCCCTTCAGGGCGACCACGGCAAGCCGCGTCCCGCGCTTGTGATCCAATCCGA
CCGGTTTCCGGACACCGCCACCACGACCGTCCTTCTGGTTACATCCACCTTGGTGGATGCGCCGTTGATTCGGCTGACGG
TCGAACCCTCTGCGGAGAACGGGTTGCGTATGCGATCACAGATCATGATCGACAAAGCCGTGACAGTTCGAACGGACAAG
CTGGGGAAAGCTTTCGGTCAGCTGGACGATACCGCCATGATCGAGGTGAACCGGGCACTAGCACTGTTTTTAGGAGTGGC
GGGGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10148.65 Da        Isoelectric Point: 10.1733

>AT183476 WP_048264996.1 NZ_CP065834:228286-228555 [Klebsiella pneumoniae]
MSALRKQRIDLRLTDDDKSLIEEAAAMSNQTITQFVVASASERAAEVIEQHRRMVLNEESWNLVMDAIANPAAPNERLKR
AAKRLQKMR

Download         Length: 270 bp

>AT183476 NZ_CP087044:170756-170974 [Pseudosulfitobacter pseudonitzschiae]
ATGCCAACCTCTGTTTCTCACCGTGTCCAGAAGCGTCGCGATGCCTTGCGTGCCGCTGGTTTGCGCCCCGTCCAGATTTG
GGTGCCGGATACCCGCCGCCCCGGTTTCGAGGCTGAGTGCCGCCGCCAGGCTGCAATTGTAGCCAAGGCCGATCGCGCAG
ATGATACTCTGAACGATTTCCTGGATGCCTCCCTGGTCGATGTGGATGACTGGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References