Detailed information of TA system TA183475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 19857..20608
• Replicon: plasmid p1
• Accession: NZ_CP065834
• Organism: Klebsiella pneumoniae strain KpC5

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: I8N78_RS25100
• Protein ID: WP_106510830.1
• Coordinates: 20126..20608 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: I8N78_RS25095
• Protein ID: WP_004902250.1
• Coordinates: 19857..20135 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N78_RS25075	15187..16071	-	885	WP_004186900.1	hypothetical protein	-
I8N78_RS25080	17323..17781	+	459	WP_173675456.1	hypothetical protein	-
I8N78_RS25085	18024..18338	-	315	WP_040210113.1	hypothetical protein	-
I8N78_RS25090	19523..19735	+	213	WP_016946198.1	hypothetical protein	-
I8N78_RS25095	19857..20135	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
I8N78_RS25100	20126..20608	+	483	WP_106510830.1	GNAT family N-acetyltransferase	Toxin
I8N78_RS25105	20792..21489	+	698	Protein_26	IS1 family transposase	-
I8N78_RS25110	21764..22363	+	600	WP_032437781.1	XRE family transcriptional regulator	-
I8N78_RS25115	22374..23144	+	771	WP_032437782.1	ATP-binding cassette domain-containing protein	-
I8N78_RS25120	23181..23945	+	765	WP_110226979.1	transporter substrate-binding domain-containing protein	-
I8N78_RS25125	23951..24640	+	690	WP_110226980.1	ABC transporter permease subunit	-
I8N78_RS25130	24637..25329	+	693	WP_032437785.1	ABC transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fyuA / ybtE / ybtT / ybtU / irp1 / irp2 / ybtA / ybtP / ybtQ / ybtX / ybtS	1..301560	301560
	-	flank	IS/Tn	-	-	20986..21489	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17752.48 Da
• Isoelectric Point: 8.6269

>T183475 WP_106510830.1 NZ_CP065834:20126-20608 [Klebsiella pneumoniae]
MGMREPESLTSEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWSGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T183475 NZ_CP087043:c157739-157488 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT183475 WP_004902250.1 NZ_CP065834:19857-20135 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT183475 NZ_CP087043:c157491-157225 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3