Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5028675..5029300 Replicon chromosome
Accession NZ_CP065833
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I8N78_RS24610 Protein ID WP_002882817.1
Coordinates 5028675..5029058 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I8N78_RS24615 Protein ID WP_004150355.1
Coordinates 5029058..5029300 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS24595 5026041..5026943 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I8N78_RS24600 5026940..5027575 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I8N78_RS24605 5027572..5028501 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I8N78_RS24610 5028675..5029058 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I8N78_RS24615 5029058..5029300 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I8N78_RS24620 5029505..5030422 + 918 WP_080893931.1 alpha/beta hydrolase -
I8N78_RS24625 5030436..5031377 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I8N78_RS24630 5031422..5031859 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I8N78_RS24635 5031856..5032716 - 861 WP_002882807.1 virulence factor BrkB family protein -
I8N78_RS24640 5032710..5033309 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T183474 WP_002882817.1 NZ_CP065833:c5029058-5028675 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T183474 NZ_CP087043:c157491-157225 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT183474 WP_004150355.1 NZ_CP065833:c5029300-5029058 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT183474 NZ_CP087043:c157739-157488 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References