Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4444450..4445260 Replicon chromosome
Accession NZ_CP065833
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag I8N78_RS21850 Protein ID WP_004178461.1
Coordinates 4444450..4444983 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag I8N78_RS21855 Protein ID WP_002887278.1
Coordinates 4444994..4445260 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS21845 4443281..4444402 + 1122 WP_002887282.1 cupin domain-containing protein -
I8N78_RS21850 4444450..4444983 - 534 WP_004178461.1 GNAT family N-acetyltransferase Toxin
I8N78_RS21855 4444994..4445260 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
I8N78_RS21860 4445363..4446796 - 1434 WP_020804438.1 Cu(+)/Ag(+) sensor histidine kinase -
I8N78_RS21865 4446786..4447469 - 684 WP_020804437.1 copper response regulator transcription factor CusR -
I8N78_RS21870 4447641..4449026 + 1386 WP_069197132.1 efflux transporter outer membrane subunit -
I8N78_RS21875 4449044..4449388 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T183471 WP_004178461.1 NZ_CP065833:c4444983-4444450 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T183471 NZ_CP087041:1173-1448 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT183471 WP_002887278.1 NZ_CP065833:c4445260-4444994 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT183471 NZ_CP087041:887-1180 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References