Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2597344..2598932 Replicon chromosome
Accession NZ_CP065833
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name hipA Uniprot ID A6T928
Locus tag I8N78_RS12820 Protein ID WP_004176197.1
Coordinates 2597344..2598666 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag I8N78_RS12825 Protein ID WP_002904790.1
Coordinates 2598666..2598932 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS12805 2592409..2594901 + 2493 WP_126438606.1 fimbrial biogenesis outer membrane usher protein -
I8N78_RS12810 2594889..2595893 + 1005 WP_040241874.1 type 1 fimbrial protein -
I8N78_RS12815 2596035..2597327 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
I8N78_RS12820 2597344..2598666 - 1323 WP_004176197.1 type II toxin-antitoxin system HipA family toxin Toxin
I8N78_RS12825 2598666..2598932 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
I8N78_RS12830 2599197..2599523 - 327 WP_002904788.1 hydroxyisourate hydrolase -
I8N78_RS12835 2599520..2600020 - 501 WP_023285113.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
I8N78_RS12840 2599998..2601377 - 1380 WP_004184021.1 purine permease -
I8N78_RS12845 2601723..2602877 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
I8N78_RS12850 2602858..2603781 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2418814..2639475 220661


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49111.41 Da        Isoelectric Point: 7.5357

>T183468 WP_004176197.1 NZ_CP065833:c2598666-2597344 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T183468 NZ_CP087038:c70374-69952 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT183468 WP_002904790.1 NZ_CP065833:c2598932-2598666 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT183468 NZ_CP087038:c70626-70378 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6T928

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References