Detailed information of TA system TA183456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53669..54405
• Replicon: plasmid p1
• Accession: NZ_CP065832
• Organism: Klebsiella pneumoniae strain KpC11

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I8N76_RS27160
• Protein ID: WP_003026803.1
• Coordinates: 53923..54405 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I8N76_RS27155
• Protein ID: WP_003026799.1
• Coordinates: 53669..53935 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N76_RS27110	49731..50093	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I8N76_RS27115	50143..50493	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I8N76_RS27120	50851..51120	+	270	WP_004152102.1	hypothetical protein	-
I8N76_RS27125	51108..51683	+	576	WP_004152103.1	hypothetical protein	-
I8N76_RS27130	51714..52208	+	495	WP_004152104.1	DNA-binding protein	-
I8N76_RS27135	52252..52620	+	369	WP_004152105.1	hypothetical protein	-
I8N76_RS27140	52654..52857	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I8N76_RS27145	52906..53163	+	258	WP_004152107.1	hypothetical protein	-
I8N76_RS27150	53239..53493	+	255	WP_004152108.1	hypothetical protein	-
I8N76_RS27155	53669..53935	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I8N76_RS27160	53923..54405	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I8N76_RS27165	54564..55818	-	1255	Protein_56	IS3 family transposase	-
I8N76_RS27170	55906..56868	-	963	WP_023313934.1	M48 family metalloprotease	-
I8N76_RS27175	56855..57604	-	750	WP_009483782.1	phosphate-starvation-inducible PsiE family protein	-
I8N76_RS27180	57842..58039	-	198	WP_004152115.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..202315	202315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T183456 WP_003026803.1 NZ_CP065832:53923-54405 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T183456 NZ_CP087025:1337350-1337604 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT183456 WP_003026799.1 NZ_CP065832:53669-53935 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT183456 NZ_CP087025:1337601-1337930 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66