Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5396054..5396679 Replicon chromosome
Accession NZ_CP065831
Organism Klebsiella pneumoniae strain KpC11

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I8N76_RS26520 Protein ID WP_002882817.1
Coordinates 5396054..5396437 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I8N76_RS26525 Protein ID WP_004150355.1
Coordinates 5396437..5396679 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N76_RS26505 5393420..5394322 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I8N76_RS26510 5394319..5394954 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I8N76_RS26515 5394951..5395880 + 930 WP_004146237.1 formate dehydrogenase accessory protein FdhE -
I8N76_RS26520 5396054..5396437 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I8N76_RS26525 5396437..5396679 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
I8N76_RS26530 5396884..5397801 + 918 WP_009309725.1 alpha/beta hydrolase -
I8N76_RS26535 5397815..5398756 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I8N76_RS26540 5398801..5399238 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
I8N76_RS26545 5399235..5400095 - 861 WP_004146232.1 virulence factor BrkB family protein -
I8N76_RS26550 5400089..5400688 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T183455 WP_002882817.1 NZ_CP065831:c5396437-5396054 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T183455 NZ_CP087024:45016-45291 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT183455 WP_004150355.1 NZ_CP065831:c5396679-5396437 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT183455 NZ_CP087024:44730-45023 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References