Detailed information of TA system TA183452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4805646..4806456
• Replicon: chromosome
• Accession: NZ_CP065831
• Organism: Klebsiella pneumoniae strain KpC11

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: I8N76_RS23785
• Protein ID: WP_048299784.1
• Coordinates: 4805646..4806179 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: I8N76_RS23790
• Protein ID: WP_002887278.1
• Coordinates: 4806190..4806456 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N76_RS23780	4804477..4805598	+	1122	WP_004214138.1	cupin domain-containing protein	-
I8N76_RS23785	4805646..4806179	-	534	WP_048299784.1	GNAT family N-acetyltransferase	Toxin
I8N76_RS23790	4806190..4806456	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
I8N76_RS23795	4806559..4807992	-	1434	WP_002887275.1	Cu(+)/Ag(+) sensor histidine kinase	-
I8N76_RS23800	4807982..4808665	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
I8N76_RS23805	4808837..4810222	+	1386	WP_040089708.1	efflux transporter outer membrane subunit	-
I8N76_RS23810	4810240..4810584	+	345	WP_023287080.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19826.65 Da
• Isoelectric Point: 5.2614

>T183452 WP_048299784.1 NZ_CP065831:c4806179-4805646 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGYDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T183452 NZ_CP087021:13247-13498 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT183452 WP_002887278.1 NZ_CP065831:c4806456-4806190 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT183452 NZ_CP087021:13495-13761 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1