Detailed information of TA system TA183440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1931931..1932521
• Replicon: chromosome
• Accession: NZ_CP065831
• Organism: Klebsiella pneumoniae strain KpC11

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: I8N76_RS09515
• Protein ID: WP_023341911.1
• Coordinates: 1932189..1932521 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: I8N76_RS09510
• Protein ID: WP_000288812.1
• Coordinates: 1931931..1932188 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N76_RS09485	1928531..1929880	-	1350	WP_077254247.1	DNA (cytosine-5-)-methyltransferase	-
I8N76_RS09490	1929888..1930304	-	417	WP_124047552.1	hypothetical protein	-
I8N76_RS09495	1930634..1931032	+	399	WP_198091672.1	ash family protein	-
I8N76_RS09500	1930932..1931393	+	462	WP_040210976.1	hypothetical protein	-
I8N76_RS09505	1931390..1931596	+	207	WP_022615589.1	helix-turn-helix domain-containing protein	-
I8N76_RS09510	1931931..1932188	+	258	WP_000288812.1	antitoxin	Antitoxin
I8N76_RS09515	1932189..1932521	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I8N76_RS09525	1932843..1934279	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
I8N76_RS09535	1934645..1936099	-	1455	WP_004148975.1	AMP nucleosidase	-
I8N76_RS09540	1936229..1936474	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T183440 WP_023341911.1 NZ_CP065831:1932189-1932521 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T183440 NZ_CP087009:c545654-545391 [Pseudosulfitobacter pseudonitzschiae]
ATGAAGTTCGAGTATGACCCTGACAAGAGTGCCGGGAACAAGGCCAAGCACGGCATCGACTTCGATGAAGCGCAAGCGCT
TTGGGAAGACCCGTGGCTGTTGGAGGCTCCCGCTCGAACAGATGACGAGCCGCGCTTTTTTGCAATAGGCATGATCAAGG
AACGGCATTGGTCAGCAGTTTGCACCTATCGCGGTGATATTATTCGGATCATCTCGGTGCGCCGCGCCAGAAAACAGGAG
ATTGAGCACTATGAAAGCACGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT183440 WP_000288812.1 NZ_CP065831:1931931-1932188 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT183440 NZ_CP087009:c545404-545189 [Pseudosulfitobacter pseudonitzschiae]
ATGAAAGCACGTGAGTTCGATCAAAAATTTGACACAGGTGAGAACGTCAGCGATCTTGTGGACTGGTCCAAAGCTAGGCG
GACGAATACCCAGACGAAGCGAGTCAATGTGGATTTTCCGTCGTGGGTCGTTTCTGGTTTGGATCAGAAGGCAAAGCAGC
TCGGCGTTACTCGTCAGTCGCTCATCAAACTCTGGATCGCCGAGCGGCTCCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0