Detailed information of TA system TA183439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1538024..1538760
• Replicon: chromosome
• Accession: NZ_CP065831
• Organism: Klebsiella pneumoniae strain KpC11

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: I8N76_RS07695
• Protein ID: WP_071004368.1
• Coordinates: 1538278..1538760 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I8N76_RS07690
• Protein ID: WP_003026799.1
• Coordinates: 1538024..1538290 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N76_RS07665	1533173..1534204	-	1032	WP_071004365.1	IS630 family transposase	-
I8N76_RS07670	1534313..1534790	+	478	Protein_1500	hypothetical protein	-
I8N76_RS07675	1534834..1536067	+	1234	Protein_1501	IS3 family transposase	-
I8N76_RS07680	1536078..1536938	+	861	WP_070083051.1	LysR family transcriptional regulator	-
I8N76_RS07685	1537176..1537729	+	554	Protein_1503	hypothetical protein	-
I8N76_RS07690	1538024..1538290	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I8N76_RS07695	1538278..1538760	+	483	WP_071004368.1	GNAT family N-acetyltransferase	Toxin
I8N76_RS07700	1539121..1539720	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
I8N76_RS07705	1539933..1540877	-	945	WP_077254249.1	fimbrial protein	-
I8N76_RS07710	1540889..1541467	-	579	WP_032432061.1	type 1 fimbrial protein	-
I8N76_RS07715	1541471..1542211	-	741	WP_048978466.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (73-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17321.89 Da
• Isoelectric Point: 9.1200

>T183439 WP_071004368.1 NZ_CP065831:1538278-1538760 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKSGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T183439 NZ_CP087008:29715-29990 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT183439 WP_003026799.1 NZ_CP065831:1538024-1538290 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT183439 NZ_CP087008:29429-29722 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66