Detailed information of TA system TA183435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53667..54403
• Replicon: plasmid p1
• Accession: NZ_CP065826
• Organism: Klebsiella pneumoniae strain KpPF25

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: I8N74_RS25245
• Protein ID: WP_003026803.1
• Coordinates: 53921..54403 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: I8N74_RS25240
• Protein ID: WP_003026799.1
• Coordinates: 53667..53933 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N74_RS25195	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
I8N74_RS25200	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
I8N74_RS25205	50849..51118	+	270	WP_004152102.1	hypothetical protein	-
I8N74_RS25210	51106..51681	+	576	WP_004152103.1	hypothetical protein	-
I8N74_RS25215	51712..52206	+	495	WP_004152104.1	DNA-binding protein	-
I8N74_RS25220	52250..52618	+	369	WP_004152105.1	hypothetical protein	-
I8N74_RS25225	52652..52855	+	204	WP_004152106.1	hemolysin expression modulator Hha	-
I8N74_RS25230	52904..53161	+	258	WP_004152107.1	hypothetical protein	-
I8N74_RS25235	53237..53491	+	255	WP_004152108.1	hypothetical protein	-
I8N74_RS25240	53667..53933	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
I8N74_RS25245	53921..54403	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
I8N74_RS25250	54615..55961	+	1347	WP_020314316.1	ISNCY family transposase	-
I8N74_RS25255	56462..57716	-	1255	Protein_57	IS3 family transposase	-
I8N74_RS25260	57804..58766	-	963	WP_004152113.1	M48 family metalloprotease	-
I8N74_RS25265	58753..59241	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(D) / blaSHV-1 / sul1 / qacE / ant(3'')-Ia / dfrA8 / catA1	-	1..213019	213019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T183435 WP_003026803.1 NZ_CP065826:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T183435 NZ_CP087003:104217-104468 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAGAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT183435 WP_003026799.1 NZ_CP065826:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT183435 NZ_CP087003:104465-104731 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66