Detailed information of TA system TA183433

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4743464..4745145
• Replicon: chromosome
• Accession: NZ_CP065825
• Organism: Klebsiella pneumoniae strain KpPF25

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: I8N74_RS23170
• Protein ID: WP_004146663.1
• Coordinates: 4743464..4744381 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: I8N74_RS23175
• Protein ID: WP_004177809.1
• Coordinates: 4744387..4745145 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N74_RS23150	4738770..4740290	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
I8N74_RS23155	4740287..4741285	+	999	WP_004186549.1	ribose ABC transporter permease	-
I8N74_RS23160	4741316..4742257	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
I8N74_RS23165	4742332..4743339	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
I8N74_RS23170	4743464..4744381	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
I8N74_RS23175	4744387..4745145	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
I8N74_RS23180	4745231..4745959	-	729	WP_004177810.1	response regulator transcription factor	-
I8N74_RS23185	4746232..4748379	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
I8N74_RS23190	4748609..4749295	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T183433 WP_004146663.1 NZ_CP065825:c4744381-4743464 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T183433 NZ_CP087002:1833962-1834216 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT183433 WP_004177809.1 NZ_CP065825:c4745145-4744387 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT183433 NZ_CP087002:1834213-1834542 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05