Detailed information of TA system TA183431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4503602..4504412
• Replicon: chromosome
• Accession: NZ_CP065825
• Organism: Klebsiella pneumoniae strain KpPF25

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: I8N74_RS22010
• Protein ID: WP_014908042.1
• Coordinates: 4503602..4504135 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: I8N74_RS22015
• Protein ID: WP_023343051.1
• Coordinates: 4504146..4504412 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N74_RS22005	4502433..4503554	+	1122	WP_012737335.1	cupin domain-containing protein	-
I8N74_RS22010	4503602..4504135	-	534	WP_014908042.1	GNAT family N-acetyltransferase	Toxin
I8N74_RS22015	4504146..4504412	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
I8N74_RS22020	4504515..4505948	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
I8N74_RS22025	4505938..4506621	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
I8N74_RS22030	4506794..4508179	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
I8N74_RS22035	4508197..4508541	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T183431 WP_014908042.1 NZ_CP065825:c4504135-4503602 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T183431 NZ_CP087000:c23473-23198 [Pseudosulfitobacter pseudonitzschiae]
GTGCCTGACCTTGAATGGAAGGCATTGGCGGTCGCGGACCTGATGGCGATTGTTGACTATATCTCGGATGACAATCCCGA
TGCCGCAATTTCCCTGATGGATGACATTCAAGACAAGGTGCTTGGCCTTCTGACGCATCCCAAATCCGGCCGCCCCGGTA
GGGTGAACGACACGCGAGAGCTGGTGATCCGGCCCAACTATATCGTCGTCTATGCTGAAAACACGGCGGCGGTCACCATT
CTGCGCGTTCTTCATGCGGTGCAGATGTGGCCCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT183431 WP_023343051.1 NZ_CP065825:c4504412-4504146 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT183431 NZ_CP087000:c23759-23466 [Pseudosulfitobacter pseudonitzschiae]
ATGTCCGCTCAAACCTCGATGATTCATGTTCGTGTAGATGATCAGCTCAAGGCAAAGGCCACGGATGCCCTGTCCGGTGT
CGGCCTGACGTTGTCGGACGCCGTGCGCATCCTGCTGACCCGCGTGGCAGCAGAGGGTGGCTTGCCCGCAGGGTTGACGG
CAAATCCTGACGCCTACGATGCCTGGTTCCGCGCCAAGGTACAGGACGCATTGGCCGACGCAGCACCGGCCAAGCCGCAC
GATCAGGTGATGCGAGACGCGCGCGCCTTGATCGACGGCAAGCGCGGTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A332NV98