Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2545606..2547194 Replicon chromosome
Accession NZ_CP065825
Organism Klebsiella pneumoniae strain KpPF25

Toxin (Protein)


Gene name hipA Uniprot ID A0A486S9S0
Locus tag I8N74_RS12535 Protein ID WP_023301940.1
Coordinates 2545606..2546928 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag I8N74_RS12540 Protein ID WP_002904790.1
Coordinates 2546928..2547194 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N74_RS12520 2540662..2543163 + 2502 WP_102047204.1 fimbrial biogenesis outer membrane usher protein -
I8N74_RS12525 2543151..2544155 + 1005 WP_009308876.1 type 1 fimbrial protein -
I8N74_RS12530 2544297..2545589 + 1293 WP_016946381.1 glycoside hydrolase family 10 protein -
I8N74_RS12535 2545606..2546928 - 1323 WP_023301940.1 type II toxin-antitoxin system HipA family toxin Toxin
I8N74_RS12540 2546928..2547194 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
I8N74_RS12545 2547460..2547786 - 327 WP_004143476.1 hydroxyisourate hydrolase -
I8N74_RS12550 2547783..2548283 - 501 WP_040182039.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
I8N74_RS12555 2548261..2549640 - 1380 WP_004176204.1 purine permease -
I8N74_RS12560 2549986..2551140 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
I8N74_RS12565 2551121..2552044 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49185.49 Da        Isoelectric Point: 7.5358

>T183428 WP_023301940.1 NZ_CP065825:c2546928-2545606 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T183428 NZ_CP086996:c166118-165867 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT183428 WP_002904790.1 NZ_CP065825:c2547194-2546928 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT183428 NZ_CP086996:c165870-165604 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486S9S0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References